Proteomics

Dataset Information

0

I.e. mouse ovary palmitoylation protein LC-MSMS


ABSTRACT: To identify a role for protein palmitoylation in ovarian of DHEA-induced PCOS mice model. First, we used DHEA to induce a PCOS mice model,hematoxylin-eosin (H&E) staining sections form the ovaries of the Control and DHEA groups.The mice oral gavage with DHEA disrupted ovarian morphology,the estrous cycle, and hormone profilein DHEA-induced mouse. The three pairs of ovary for each tube are mixed together as a group were lysed and divided into 2 fractions. The -HAM condition served as a negative control and the +HAM sample positive control.In the +HAM sample, the palmitate residue was cleaved off and exchanged with biotin. After the ABE reaction was completed, streptavidin beads were used to enrich for biotinylated proteins. Proteins enriched from +HAM and −HAM conditions were identified using mass spectrometry (MS).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Ovary

DISEASE(S): Polycystic Ovary Syndrome

SUBMITTER: Tang Ting  

LAB HEAD: Bing Yao

PROVIDER: PXD028835 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Rawdata_1.tar.gz Other
Rawdata_2.tar.gz Other
Rawdata_3.tar.gz Other
checksum.txt Txt
Items per page:
1 - 4 of 4
altmetric image

Publications

PPT1 regulation of HSP90α depalmitoylation participates in the pathogenesis of hyperandrogenism.

Xue Tongmin T   Zhao Shanmeizi S   Zhang Hong H   Tang Ting T   Zheng Lu L   Jing Jun J   Ge Xie X   Ma Rujun R   Ma Jinzhao J   Ren Xiaoyan X   Jueraitetibaike Kadiliya K   Guo Zhigang Z   Chen Li L   Yao Bing B  

iScience 20230203 3


Ovarian granulosa cells (GCs) in the follicle are the important mediator of steroidogenesis and foster oocyte maturation. Evidences suggested that the function of GCs could be regulated by <i>S</i>-palmitoylation. However, the role of <i>S</i>-palmitoylation of GCs in ovarian hyperandrogenism remains elusive. Here, we demonstrated that the protein from GCs in ovarian hyperandrogenism phenotype mouse group exhibits lower palmitoylation level compared with that in the control group. Using <i>S</i>  ...[more]

Similar Datasets

2012-11-06 | E-GEOD-34526 | biostudies-arrayexpress
2024-10-02 | PXD044134 | Pride
2013-06-05 | E-GEOD-40400 | biostudies-arrayexpress
2014-01-03 | E-GEOD-43266 | biostudies-arrayexpress
2023-07-05 | GSE236248 | GEO
2023-10-27 | PXD045753 | Pride
2023-02-02 | MTBLS6736 | MetaboLights
2019-03-01 | E-MTAB-6672 | biostudies-arrayexpress
2019-03-22 | PXD012731 | Pride
2017-06-01 | GSE83220 | GEO