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Mouse sperm protamine mass spectrometry

ABSTRACT: Conventional dogma presumes that protamine-mediated DNA compaction in sperm is achieved by passive electrostatics between DNA and the arginine-rich core of protamines. However, phylogenetic analysis reveals several non-arginine residues that are conserved within, but not across, species. The functional significance of these residues or post-translational modifications are poorly understood. In this experiment, we investigated the presence of post-translational modifications on mouse protamine 1 to begin to dissect their function in vivo.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Sperm

SUBMITTER: Lindsay Moritz

LAB HEAD: Sue Hammoud

PROVIDER: PXD028917 | Pride | 2023-05-17

REPOSITORIES: Pride

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			Action	DRS
	MSB38029Chym021019.raw	Raw
	MSB38029Chym021019.raw_20190212_Byonic.mzid.mzid.gz	Mzid
	MSB38029Chym021019.raw_20190212_Byonic.mzid.mzid_MSB38029Chym021019.raw_20190212_Byonic.mzid.MGF	Mzid
	MSB38029LysC021019.raw	Raw
	MSB38029LysC021019.raw_20190212_Byonic.mzid.mzid.gz	Mzid

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