Dataset Information

ChIRP-MS of long non-coding RNA HOTAIR

ABSTRACT: Accumulating evidence highlights the role of long non-coding RNAs (lncRNA) in cellular homeostasis, and their dysregulation in disease settings. Most lncRNAs function by interacting with proteins or protein complexes. While several orthogonal methods have been developed to identify these proteins, each method has its inherent strengths and limitations. Here, we combine two RNA-centric methods ChIRP-MS and RNA-BioID to obtain a comprehensive list of proteins that interact with the well-known lncRNA HOTAIR. Overexpression of HOTAIR has been associated with a metastasis-promoting phenotype in various cancers. Although HOTAIR is known to bind with PRC2 and LSD1 protein complexes, an unbiased and comprehensive method to map its interactome has not yet been performed. Both ChIRP-MS and RNA-BioID data sets show an association of HOTAIR with mitoribosomes, suggesting HOTAIR has functions independent of its (post-)transcriptional mode-of-action.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Louis Delhaye

LAB HEAD: Sven Eyckerman

PROVIDER: PXD029057 | Pride | 2022-02-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	B004598_Ap_CMB-327_SVEYC-LouisDelhaye-APMS_LacZ-her1.raw	Raw
	B004600_Ap_CMB-327_SVEYC-LouisDelhaye-APMS_LacZ-her2.raw	Raw
	B004602_Ap_CMB-327_SVEYC-LouisDelhaye-APMS_LacZ-her3.raw	Raw
	B004604_Ap_CMB-327_SVEYC-LouisDelhaye-APMS_HOTAIR-her1.raw	Raw
	B004606_Ap_CMB-327_SVEYC-LouisDelhaye-APMS_HOTAIR-her2.raw	Raw

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Publications

Orthogonal proteomics methods to unravel the HOTAIR interactome.

Delhaye Louis L De Bruycker Edith E Volders Pieter-Jan PJ Fijalkowska Daria D De Sutter Delphine D Degroeve Sven S Martens Lennart L Mestdagh Pieter P Eyckerman Sven S

Scientific reports 20220127 1

Accumulating evidence highlights the role of long non-coding RNAs (lncRNAs) in cellular homeostasis, and their dysregulation in disease settings. Most lncRNAs function by interacting with proteins or protein complexes. While several orthogonal methods have been developed to identify these proteins, each method has its inherent strengths and limitations. Here, we combine two RNA-centric methods ChIRP-MS and RNA-BioID to obtain a comprehensive list of proteins that interact with the well-known lnc ...[more]

PMID: 35087108

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