Proteomics

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Rat vascular smooth muscle cells LC-MS/MS

ABSTRACT: Crotonylation of histones is discovered of late as one of post-translational modification that can regulate gene expression. However, the function of crotonylation on non-histone proteins in vascular smooth muscle cells (VSMC) is unclear. Here, we aim to use modification and proteomic analysis to find the cellular characteristic of crotonylated non-histone proteins and the crosstalk with ubiquitinated proteins in vascular smooth muscle cell (VSMC) phenotypic remodeling. We performed modification and proteomic analysis of VSMCs before and after stimulated with platelet-derived growth factor-BB (PDGF-BB). The crotonylated and ubiquitinated pan-antibody was used to enrich the protein and then subjected to high-throughput mass spectrometry analysis. The enrichment analysis was performed within differentially modified proteins in regards to GO terms, KEGG and protein domain.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture, Blood Vessel Smooth Muscle Cell

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Li-Hua Dong  

LAB HEAD: Li-Hua Dong

PROVIDER: PXD029070 | Pride | 2022-05-19

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Crotonylome_Con_rep1.raw Raw
Crotonylome_Con_rep2.raw Raw
Crotonylome_Con_rep3.raw Raw
Crotonylome_PDGF_rep1.raw Raw
Crotonylome_PDGF_rep2.raw Raw
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Publications

Dynamics and Functional Interplay of Nonhistone Lysine Crotonylome and Ubiquitylome in Vascular Smooth Muscle Cell Phenotypic Remodeling.

Cao Shan-Hu SH   Chen Zhi-Huan ZH   Ma Ru-Yuan RY   Yue Lin L   Jiang Han-Mei HM   Dong Li-Hua LH  

Frontiers in cardiovascular medicine 20220308

<h4>Background</h4>The crotonylation of histones is discovered of late as one of the post-translational modifications (PTMs) that can regulate gene expression. However, the function of crotonylation on nonhistone proteins in vascular smooth muscle cells (VSMCs) is unclear. Here, we aim to find the cellular characteristics of crotonylated nonhistone proteins and the cross talk with ubiquitinated proteins in VSMC phenotypic remodeling using the modified omics and proteomic analysis.<h4>Methods</h4  ...[more]

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