Proteomics

Dataset Information

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Searching for interactors of endogenous SLX4

ABSTRACT: The project was aimed at identifying binding partners of endogenous SLX4 in HeLa cells. Whole cell extracts from HeLa Flp-In T-Rex cells were used for immunoprecipitation performed with anti-SLX4 antibodies from Bethyl Laboratories (A302-270A and A302-269A), either used alone or in combination. To discriminate bona fide SLX4 partners from non-specific proteins recognized by the anti-SLX4 antibodies, cells were transfected with control (Luc) siRNA or a siRNA targeting SLX4. After immunoprecipitation, protein complexes were analysed by LC-MSMS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: AUDEBERT Stephane  

LAB HEAD: Pierre-Henri Gaillard

PROVIDER: PXD029084 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Exp1_siLuc.msf Msf
Exp1_siLuc.raw Raw
Exp1_siLuc_20200202001339.msf Msf
Exp1_siLuc_20200202001339.raw Raw
Exp1_siLuc_20200202024939.msf Msf
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Publications

SLX4 dampens MutSα-dependent mismatch repair.

Guervilly Jean-Hugues JH   Blin Marion M   Laureti Luisa L   Baudelet Emilie E   Audebert Stéphane S   Gaillard Pierre-Henri PH  

Nucleic acids research 20220301 5

The tumour suppressor SLX4 plays multiple roles in the maintenance of genome stability, acting as a scaffold for structure-specific endonucleases and other DNA repair proteins. It directly interacts with the mismatch repair (MMR) protein MSH2 but the significance of this interaction remained unknown until recent findings showing that MutSβ (MSH2-MSH3) stimulates in vitro the SLX4-dependent Holliday junction resolvase activity. Here, we characterize the mode of interaction between SLX4 and MSH2,  ...[more]

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