Dataset Information

Proteomics Analysis of Bacterial Chondronecrosis with Osteomyelitis in Broiler Chickens

ABSTRACT: Proteomic analysis was performed on the proximal tibia head from healhty and BCO-affected broiler (meat-type) chickens using Bruker Daltonics amaZon series Mass Spectrometer. This analysis was undertaken to identify key protein signature involved in BCO, a common cause of lameness.

INSTRUMENT(S): Bruker Daltonics amaZon series

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Long Bone

DISEASE(S): Lameness

SUBMITTER: Sami Dridi

LAB HEAD: Sami Dridi

PROVIDER: PXD029085 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	281474976712143__74163_N1_4_T_1_01_472_.mzid.gz	Mzid
	281474976712143__74163_N1_4_T_1_01_472_.mzid_281474976712143__74163_N1_4_T_1_01_472_.MGF	Mzid
	281474976712144__74163_N2_4_T_2_01_473_.mzid.gz	Mzid
	281474976712144__74163_N2_4_T_2_01_473_.mzid_281474976712144__74163_N2_4_T_2_01_473_.MGF	Mzid
	281474976712145__74163_N3_4_T_3_01_474_.mzid.gz	Mzid

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Publications

Comparative- and network-based proteomic analysis of bacterial chondronecrosis with osteomyelitis lesions in broiler's proximal tibiae identifies new molecular signatures of lameness.

Cook Jennifer J Greene Elizabeth S ES Ramser Alison A Mullenix Garrett G Dridi Jalila S JS Liyanage Rohana R Wideman Robert R Dridi Sami S

Scientific reports 20230412 1

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a specific cause of lameness in commercial fast-growing broiler (meat-type) chickens and represents significant economic, health, and wellbeing burdens. However, the molecular mechanisms underlying the pathogenesis remain poorly understood. This study represents the first comprehensive characterization of the proximal tibia proteome from healthy and BCO chickens. Among a total of 547 proteins identified, 222 were differentially expressed (DE) ...[more]

PMID: 37045932

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Project description:Background In broilers, heat stress can result in reduced feed consumption, digestive inefficiency, impaired metabolism, and even death. The broiler sector of the U.S. poultry industry incurs approximately $52 million in heat stress-related losses annually. The objective of this study is to characterize the effects of chronic, cyclic heat stress on the transcriptome of a metabolically active organ, the liver. Characterizing the liver transcriptome of heat-stressed broilers will help clarify the effects of heat stress on metabolism. This information will provide a platform for future investigations that further elucidate physiologic responses to heat stress and seek methods to ameliorate the negative impacts of heat. Results Transcriptome sequencing of the livers of 8 broiler males using Illumina HiSeq 2000 technology, resulted in a total of 138 million, 100 base pair single end reads, yielding 13.8 gigabases of sequence. Forty genes were differentially expressed at a significance level of P-value < 0.05 and a fold change ≥ 2 in response to chronic, cyclic heat stress (mid-point of the last day of a 7-day cyclic heat stress of 7 hours per day), with 27 down-regulated and 13 up-regulated. Two gene networks were created from the function-based Ingenuity Pathway Analysis (IPA) of the differentially expressed genes; “Cell Signaling, Molecular Transport, Small Molecule Biochemistry” and “Endocrine System Development and Function, Small Molecule Biochemistry Cell Signaling”. Members of the MAPK signaling pathway and differentially expressed genes that are associated with MAPK-related functions were prominent in the networks. Cellular proliferation and differentiation, inflammationand stress-related signaling, and apoptosis-associated genes were down-regulated in response to heat stress. Genes responsible for inhibiting feed intake and sphingolipidrelated signaling were up-regulated. Genes involved with the regulation of inflammation, stress, thyroid hormone level, and body temperature were both up- and down-regulated. Conclusions Chronic, cyclic heat stress of broilers results in metabolic changes that can be characterized through RNA-seq analysis of the liver transcriptome. The primary affected pathways included cell signaling, molecular transport, endocrine system development and signaling, and small molecule biochemistry. Examination of 2 heat treatments. Four heat stressed liver samples and 4 control liver samples analyzed.