Proteomics

Dataset Information

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Sequestosome 1 is part of the interaction network of VAPB


ABSTRACT: VAPB (Vesicle-Associated-membrane Protein-associated proteins B) is a tail-anchored mem-brane protein of the endoplasmic reticulum that can also be detected at the inner nuclear mem-brane. As a component of many contact sites between the endoplasmic reticulum and other or-ganelles, VAPB is engaged in multiple protein interactions with a plethora of binding partners. A mutant version of VAPB, P56S-VAPB, that results from a single point mutation is involved in a familial form of amyotrophic lateral sclerosis (ALS8). We performed RAPIDS (rapamycin- and APEX-dependent identification of proteins by SILAC) to identify proteins that interact with or are in close proximity to P56S-VAPB. The mutation abrogates the interaction of VAPB with many known binding partners. Instead, Sequestosome 1 (SQSTM1) was identified as a major in-teraction/proximity partner, a protein that is known as an autophagic adapter. Remarkably, not only the mutant P56S-VAPB, but also the wild type protein interacts with SQSTM1, as shown by proximity ligation assays and co-immunoprecipiation experiments.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christof Lenz  

LAB HEAD: Christof Lenz

PROVIDER: PXD029113 | Pride | 2022-02-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
C_James_281019.zip Other
C_James_281019_261119_R1_L1_01.raw Raw
C_James_281019_261119_R1_L1_02.raw Raw
C_James_281019_261119_R1_L1_03.raw Raw
C_James_281019_261119_R1_L1_04.raw Raw
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Publications

Sequestosome 1 Is Part of the Interaction Network of VAPB.

James Christina C   Lenz Christof C   Urlaub Henning H   Kehlenbach Ralph H RH  

International journal of molecular sciences 20211209 24


VAPB (Vesicle-Associated-membrane Protein-associated protein B) is a tail-anchored membrane protein of the endoplasmic reticulum that can also be detected at the inner nuclear membrane. As a component of many contact sites between the endoplasmic reticulum and other organelles, VAPB is engaged in multiple protein interactions with a plethora of binding partners. A mutant version of VAPB, P56S-VAPB, which results from a single point mutation, is involved in a familial form of amyotrophic lateral  ...[more]

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