Proteomics

Dataset Information

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The IPF fibroblastic focus is an active collagen biosynthesis factory embedded in a distinct extracellular matrix


ABSTRACT: The Fibroblastic Focus (FF) is a lesion typical of Idiopathic Pulmonary Fibrosis (IPF). It is the site of myofibroblast accumulation and extracellular matrix (ECM) production. The molecular composition and function of the FF and surrounding tissue remain undefined. Utilizing laser capture microdissection coupled mass spectrometry (LCM-MS), we interrogate the FF, adjacent mature scar tissue, and adjacent alveoli in 6 IPF specimens. To serve as a control, we perform LCM-MS on alveoli from 6 non-fibrotic controls.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

DISEASE(S): Idiopathic Pulmonary Fibrosis

SUBMITTER: Craig Lawless  

LAB HEAD: Jeremy Herrera

PROVIDER: PXD029341 | Pride | 2022-08-31

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
HLF2_NmlAlv_cond.raw Raw
HLF3401_NmlAlv.raw Raw
HLF3535_NmlAlv.raw Raw
HLF3999_NmlAlv.raw Raw
HLF3_NmlAlv.raw Raw
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Publications

The UIP/IPF fibroblastic focus is a collagen biosynthesis factory embedded in a distinct extracellular matrix.

Herrera Jeremy A JA   Dingle Lewis L   Montero M Angeles MA   Venkateswaran Rajamiyer V RV   Blaikley John F JF   Lawless Craig C   Schwartz Martin A MA  

JCI insight 20220822 16


Usual interstitial pneumonia (UIP) is a histological pattern characteristic of idiopathic pulmonary fibrosis (IPF). The UIP pattern is patchy with histologically normal lung adjacent to dense fibrotic tissue. At this interface, fibroblastic foci (FF) are present and are sites where myofibroblasts and extracellular matrix (ECM) accumulate. Utilizing laser capture microdissection-coupled mass spectrometry, we interrogated the FF, adjacent mature scar, and adjacent alveoli in 6 fibrotic (UIP/IPF) s  ...[more]

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