Comparative Analysis Reveals Genotype-specific Lyisne Malonylation of Toxoplasma gondii

ABSTRACT: Toxoplasma gondii is a protozoan parasite which can infect a wide range of animals, including humans. According to virulence, it is generally divided into three different strains, namely type I (RH strain), type II (PRU strain) and type III (VEG strain). Lysine malonylation (Kmal) is a new type of post-translational modification (PTM), which has been reported to regulate diverse biological pathways in various organisms, including T. gondii. However, there is no knowledge about whether lysine malonylation regulates the virulence of different strains in T. gondii. In this study, for the first time, we identified and quantified lysine malonylation level in three strains of T. gondii. In total, 111 proteins and 152 sites were up-regulated, 17 proteins and sites were down-regulated in RH compared with PRU strains, respectively; 50 proteins and 59 sites were up-regulated, 50 proteins and 53 sites were down-regulated in RH strain compared with VEG strains; 72 proteins and 90 sites were up-regulated, 7 proteins and 8 sites were down-regulated in VEG strain compared with PRU strains. Further analysis indicates that these proteins are involved in many important biological processes and regulating virulence-related functions of T. gondii. These findings provide novel and important resource for the role of lysine malonytion in virulence of T. gondii and provide a new direction for the research of vaccine in T. gondii.

INSTRUMENT(S): Bruker Daltonics BioTOF series

ORGANISM(S): Toxoplasma Gondii Veg

SUBMITTER: Lan-Be Nie  

LAB HEAD: xing-quan zhu

PROVIDER: PXD029366 | Pride | 2022-05-11

REPOSITORIES: Pride