Proteomics

Dataset Information

0

MTOR inhibition via Rapamycin treatment partially reverts the deficit in energy metabolism caused by FH loss in RPE cells


ABSTRACT: To obtain insight into the molecular mechanism of FH intracellular function in hTERT-RPE1 cells, we analyzed the interactome of FH via its immunoprecipitation followed by MS-based analysis. We found that FH interacts with essential components of the ubiquitin-proteasomal pathway (UPS) as well as with RB1/E2F signalling in a complement-pathway independent manner. Moreover, we found, that FH silencing affects mRNA levels of the E3 Ubiquitin-Protein Ligase Parkin and PTEN induced putative kinase (Pink1), both of them are associated with UPS. As inhibition of mTORC1 has been previoulsy shown to result in increased overall protein degradation via UPS and as FH mRNA and protein levels were shown to be affected by inhibition of UPS, our data stress a potential regulatory link between endogenous FH activity and UPS.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Retinal Pigment Epithelial Cell, Cell Culture

DISEASE(S): Age Related Macular Degeneration

SUBMITTER: Mohamed Ali Jarboui  

LAB HEAD: Angela Armento

PROVIDER: PXD029408 | Pride | 2022-02-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CFH_ctr_Lys_1.raw Raw
CFH_ctr_Lys_2.raw Raw
CFH_ctr_Lys_3.raw Raw
CFH_ctr_Lys_4.raw Raw
IGG_Lys_1.raw Raw
Items per page:
1 - 5 of 10
altmetric image

Publications

mTOR Inhibition via Rapamycin Treatment Partially Reverts the Deficit in Energy Metabolism Caused by FH Loss in RPE Cells.

Merle David A DA   Provenzano Francesca F   Jarboui Mohamed Ali MA   Kilger Ellen E   Clark Simon J SJ   Deleidi Michela M   Armento Angela A   Ueffing Marius M  

Antioxidants (Basel, Switzerland) 20211203 12


Age-related macular degeneration (AMD) is a complex degenerative disease of the retina with multiple risk-modifying factors, including aging, genetics, and lifestyle choices. The combination of these factors leads to oxidative stress, inflammation, and metabolic failure in the retinal pigment epithelium (RPE) with subsequent degeneration of photoreceptors in the retina. The alternative complement pathway is tightly linked to AMD. In particular, the genetic variant in the complement factor H gene  ...[more]

Similar Datasets

2022-06-29 | E-MTAB-11137 | biostudies-arrayexpress
2022-10-26 | GSE213689 | GEO
2010-05-11 | GSE21760 | GEO
2012-06-12 | E-GEOD-13691 | biostudies-arrayexpress
2010-05-28 | E-GEOD-21760 | biostudies-arrayexpress
2009-01-29 | GSE10316 | GEO
2009-02-09 | E-GEOD-10316 | biostudies-arrayexpress
2012-06-13 | GSE13691 | GEO
2021-03-08 | PXD023751 | Pride
2012-07-31 | E-GEOD-39771 | biostudies-arrayexpress