Proteomics

Dataset Information

0

Proteome and phosphoproteome analysis of autophagy deficient ATG5flox:CamKII-Cre and ATG5flox:VGAT-Cre conditional knockout mice

ABSTRACT: The experiment was performed to find selective targets of autophagy in different subtypes of neurons. Therefore, brain tissue of conditional knockout mice lacking autophagy protein ATG5 in inhibitory or excitatory neurons was used. Cortices (ATG5flox:CamKII-Cre) / striata (ATG5flox:VGAT-Cre) of mice were mixed with lys6 labeled brain lysates as internal controls between the samples. Samples were measured by LC-MS/MS and resulting H/L ratios were used to quantify proteomic and phosphoproteomic changes.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Frederik Tellkamp  

LAB HEAD: Natalia L Kononenko

PROVIDER: PXD029435 | Pride | 2022-11-15

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
MQ_output.7z Other
MQ_parameter_file.xml Xml
MaxQuant_1.6.14.0.zip Other
Raw_files.7z Other
experimentalDesign.xlsx Xlsx
Items per page:
1 - 5 of 5
altmetric image

Publications

Autophagy provides nutrients during starvation and eliminates detrimental cellular components. However, accumulating evidence indicates that autophagy is not merely a housekeeping process. Here, by combining mouse models of neuron-specific ATG5 deficiency in either excitatory or inhibitory neurons with quantitative proteomics, high-content microscopy, and live-imaging approaches, we show that autophagy protein ATG5 functions in neurons to regulate cAMP-dependent protein kinase A (PKA)-mediated p  ...[more]

Similar Datasets

Proteomics Global proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons
2022-11-15 | PXD031535 | Pride
Proteomics Detection of putative PKA target phosphorylation sites in Atg5flox:CamKII-Cre wildtype and knockout mice brain tissue
2022-11-15 | PXD031106 | Pride
Proteomics Global proteome of Atg5flox:CamKIIα-Cre:tdTomato FACS sorted WT and KO neurons
2022-11-15 | PXD031552 | Pride
Proteomics Global proteome of Atg5flox:CAG-CreTmx WT and KO neurons
2022-11-15 | PXD031487 | Pride
Clinical An observational study of intestinal CRE screening in patients with hematologic malignancies with high risk factors for treatment decisions
| 2699279 | ecrin-mdr-crc
Proteomics Quantitative proteomic analysis of the livers from 5-week-old HyD-LIRflox/flox and HyD-LIRflox/flox; Alb-Cre mice.
2023-04-24 | PXD037013 | JPOST Repository
Transcriptomics Expression in IFN-gamma treated control and Atg5-/- macrophages
2012-01-05 | E-GEOD-34863 | biostudies-arrayexpress
Transcriptomics Subversion of host innate immunity by Rickettsia australis via a modified autophagic response in macrophages
2021-04-06 | GSE171160 | GEO
Genomics CRE-W
| PRJNA857880 | ENA
Proteomics Glomerular and tubule OST48 Podocin-Cre SWATH
2021-07-26 | PXD011434 | Pride