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Detection of the partially purified 2-phenanthroate:CoA ligase of TRIP

ABSTRACT: The 2-phenanthroate:CoA ligase from the sulfate reducing bacterium Desulfatiglans TRIP_1 was partially purified using ammonium sulfate precipitation and hydrophobic interaction chromatography. Due to low biomass and the long growth period of the culture, no further purification steps were possible. The partially purified sample was used to identify which of the three bioinfomatically determined genes of the CoA-ligase is the one that converts 2-phenanthroic acid to 2-phenanthroyl-CoA.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Uncultured Desulfatiglans Sp.

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD029438 | Pride | 2024-09-05

REPOSITORIES: Pride

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			Action	DRS
	ACE_0608_IK01.raw	Raw
	ACE_0608_IK02.raw	Raw
	ACE_0608_IK03.raw	Raw
	ACE_0608_IK04.raw	Raw
	ACE_0608_IK05.raw	Raw

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