Proteomics

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Altered proteome in translationinitiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage

ABSTRACT: The recognition of AUG start codon and selection of the open reading frame (ORF) is fundamental to protein biosynthesis. Defect in the start codon selection adversely affectproteome and have a pleiotropic effect on cellular function. Using proteomic techniques, we identified differential protein expression patterns in the translation initiation defectivehyper GTPaseeIF5G31R mutant cells. Consistently, we observed altered proteome involved in protein catabolism, nucleotide metabolism, fatty acid metabolism, glycolysis, and autophagy.The utilization of the upstream UUG codons by the eIF5G31R mutation caused downregulation of uridylate kinase expression and DNA damage. The eIF5G31R mutant cells showed lower Glutathione levels, high ROS activity, and sensitivity to H2O2.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Pankaj Alone  

LAB HEAD: Pankaj V Alone

PROVIDER: PXD029460 | Pride | 2022-05-19

REPOSITORIES: Pride

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Dataset's files

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Action DRS
173_C8.dat Other
173_C8.mgf Mgf
173_C8.xml Xml
188_C11.dat Other
188_C11.mgf Mgf
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Publications

Altered proteome in translation initiation fidelity defective eIF5<sup>G31R</sup> mutant causes oxidative stress and DNA damage.

Ram Anup Kumar AK   Mallik Monalisha M   Reddy R Rajendra RR   Suryawanshi Amol Ratnakar AR   Alone Pankaj V PV  

Scientific reports 20220323 1

The recognition of the AUG start codon and selection of an open reading frame (ORF) is fundamental to protein biosynthesis. Defect in the fidelity of start codon selection adversely affect proteome and have a pleiotropic effect on cellular function. Using proteomic techniques, we identified differential protein abundance in the translation initiation fidelity defective eIF5<sup>G31R</sup> mutant that initiates translation using UUG codon in addition to the AUG start codon. Consistently, the eIF5  ...[more]

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