Dataset Information

Photo-affinity labeling with photo-sanglifehrin A probes

ABSTRACT: The interactome of 2 photo-Sanglifehrin A (pSfA) probes in Jurkat and K562 cells was profiled.

INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christina Woo

LAB HEAD: Christina M. Woo

PROVIDER: PXD029540 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	0419302619LHF_SAM05419_Hope389_HpH1.raw	Raw
	0419302619LHF_SAM05419_Hope389_HpH1_20190502001118.raw	Raw
	0419302619LHF_SAM05419_Hope389_HpH2.raw	Raw
	0419302619LHF_SAM05419_Hope389_HpH4.raw	Raw
	0419302619LHF_SAM05419_Hope389_HpH5.raw	Raw

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Publications

Sanglifehrin A mitigates multiorgan fibrosis by targeting the collagen chaperone cyclophilin B.

Flaxman Hope A HA Chrysovergi Maria-Anna MA Han Hongwei H Kabir Farah F Lister Rachael T RT Chang Chia-Fu CF Yvon Robert R Black Katharine E KE Weigert Andreas A Savai Rajkumar R Egea-Zorrilla Alejandro A Pardo-Saganta Ana A Lagares David D Woo Christina M CM

JCI insight 20240620 15

Pathological deposition and crosslinking of collagen type I by activated myofibroblasts drives progressive tissue fibrosis. Therapies that inhibit collagen synthesis have potential as antifibrotic agents. We identify the collagen chaperone cyclophilin B as a major cellular target of the natural product sanglifehrin A (SfA) using photoaffinity labeling and chemical proteomics. Mechanistically, SfA inhibits and induces the secretion of cyclophilin B from the endoplasmic reticulum (ER) and prevents ...[more]

PMID: 38900587

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