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Increased soluble HLA in COVID-19 present a disease-related, diverse immunopeptidome associated with T cell immunity

ABSTRACT: HLA-presented antigenic peptides are central components of T cell-based immunity in infectious disease. Beside HLA molecules on cell surfaces, soluble HLA molecules (sHLA) are released in the blood suggested to impact cellular immune responses. We demonstrated that sHLA levels were significantly increased in COVID-19 patients and convalescent individuals compared to a control cohort and positively correlated with SARS CoV-2-directed cellular immunity. Of note, patients with severe courses of COVID-19 showed reduced sHLA levels. Mass spectrometry-based characterization of sHLA-bound antigenic peptides, the so called soluble immunopeptidome, revealed a COVID-19-associated increased diversity of HLA-presented peptides and identified a naturally presented SARS-CoV-2-derived peptide from the viral nucleoprotein in the plasma of COVID-19 patients. Interestingly, sHLA serum levels directly correlated with the diversity of the soluble immunopeptidome. Together, these findings suggest an inflammation-driven release of sHLA in COVID-19, directly influencing the diversity of the soluble immunopeptidome with implications for SARS-CoV-2-directed T cell-based immunity and disease outcome.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Coronavirus Infectious Disease

SUBMITTER: Annika Nelde

LAB HEAD: Juliane S. Walz

PROVIDER: PXD029567 | Pride | 2022-11-23

REPOSITORIES: Pride

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			Action	DRS
	UPN01_class_I.csv	Csv
	UPN01_class_II.csv	Csv
	UPN01_class_II_Rep1.raw	Raw
	UPN01_class_II_Rep2.raw	Raw
	UPN01_class_II_Rep3.raw	Raw

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