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Quantitative acetylome analysis of Fusarium oxysporum

ABSTRACT: Quantitative proteome and acetylome analyses were conducted by comparing the acetylation levels of the SM to those of the NM. The collected cellular proteins were extracted, digested, and labeled by different TMTs. For acetylation profiling, the pan acetyl-lysine antibody was employed to enrich the acetylated peptides, which were then submitted into mass spectrometer (MS) for LC–MS/MS analysis. For proteome profiling, the peptide fractions were submitted into MS directly.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Fusarium Cf. Oxysporum

TISSUE(S): Mycelium

SUBMITTER: Ning Zhang

LAB HEAD: Ning Zhang

PROVIDER: PXD029583 | Pride | 2022-06-09

REPOSITORIES: Pride

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			Action	DRS
	F9746TPAc.zip	Other
	F9746TPAc_1.raw	Raw
	F9746TPAc_2.raw	Raw
	F9746TPAc_3.raw	Raw
	F9746TPAc_4.raw	Raw

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Publications

Quantitative Proteomic Analysis Reveals Important Roles of the Acetylation of ER-Resident Molecular Chaperones for Conidiation in Fusarium oxysporum.

Lv Fangjiao F Xu Yang Y Gabriel Dean W DW Wang Xue X Zhang Ning N Liang Wenxing W

Molecular & cellular proteomics : MCP 20220408 5

Fusarium oxysporum is one of the most abundant and diverse fungal species found in soils and includes nonpathogenic, endophytic, and pathogenic strains affecting a broad range of plant and animal hosts. Conidiation is the major mode of reproduction in many filamentous fungi, but the regulation of this process is largely unknown. Lysine acetylation (Kac) is an evolutionarily conserved and widespread posttranslational modification implicated in regulation of multiple metabolic processes. A total o ...[more]

PMID: 35398590

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Global Proteomic Analysis of Lysine crotonylation in The Plant Pathogen Botrytis cinerea

Project description:Lysine crotonylation (Kcr), a recently discovered post-translational modification, plays a key role in the regulation of diverse cellular processes. Botrytis cinerea is a destructive necrotrophic fungal pathogen distributed worldwide with broad ranging hosts. However, the functions of Kcr are unknown in B. cinerea or any other plant fungal pathogens. Here, we comprehensively evaluated the crotonylation proteome of B. cinerea and identified 3967 Kcr sites in 1041 proteins, of which contained in 9 types of modification motifs. Our results show that although the crotonylation were largely conserved, different organisms contained distinct crotonylated proteins with unique functions. Bioinformatic analysis demonstrated that the majority of crotonylated proteins were distributed in cytoplasm (35%), mitochondria (26%) and nucleus (22%). The identified proteins were found to be involved in various metabolic and cellular processes, such as cytoplasmic translation and structural constituent of ribosome. Particularly, 26 crotonylated proteins participated in the pathogenicity of B. cinerea, suggesting a significant role for Kcr in this process. Protein interaction network analysis demonstrated that a mass of protein interactions are regulated by crotonylation. These data represent the ﬁrst report of the crotonylome of B. cinerea and provide a good foundation for further explorations of the role of Kcr in plant fungal pathogens.

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Identification of dASF/SF2 potential mRNA targets

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Identification of B52 potential mRNA targets

Project description:Deciphering the role of alternative splicing in developmental processes relies on the identification of key genes whose expression is controlled by splicing regulators throughout growth of a whole organism. Targeting expression of five SR proteins in the developing eye of Drosophila allowed us to show that these splicing factors induce various phenotypic alterations concerning eye organogenesis and viability. Although both dASF/SF2 and B52 caused defects in ommatidia structure, only B52 impairs normal photoreceptor axons projection and neurogenesis in visual ganglia. Consistently, microarray analyses revealed that many of the B52 targets are involved in brain organogenesis and we show that their splicing profile is altered both in B52 loss and gain of function. Conversely, a large proportion of dASF/SF2 targets are involved in eye development. This differential effect argues that SR proteins confer accuracy to developmental gene-expression programs, thus ensuring tissue identity and supporting cell-lineage decisions. Keywords: genetic modification Experiment aimed at identifying B52 potential mRNA targets in the Drosophila developing eye Control: GMR X GFP-NLS trangenic larvae The 2 IP- GFP cy3 vs B52 cy5 rep.1 and IP- GFP cy5 vs B52 cy3 rep.1 samples correspond to dye-swap experiments. Two replicates (rep1 and rep2) are included.

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