Dataset Information

Hypocrates, a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives

ABSTRACT: The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 106 M-1s-1 range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, making it the first circularly permuted fluorescent protein-based redox probe for which spatial organization is determined. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Escherichia Coli

SUBMITTER: Didier Vertommen

LAB HEAD: Vsevolod V. Belousov

PROVIDER: PXD029624 | Pride | 2021-12-15

REPOSITORIES: Pride

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Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives.

Kostyuk Alexander I AI Tossounian Maria-Armineh MA Panova Anastasiya S AS Thauvin Marion M Raevskii Roman I RI Ezeriņa Daria D Wahni Khadija K Van Molle Inge I Sergeeva Anastasia D AD Vertommen Didier D Gorokhovatsky Andrey Yu AY Baranov Mikhail S MS Vriz Sophie S Messens Joris J Bilan Dmitry S DS Belousov Vsevolod V VV

Nature communications 20220110 1

The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, ...[more]

PMID: 35013284

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