Dataset Information

Sulfamethoxazole triggers specific microbial activities under aerobic heterotrophic conditions: A metaproteomic approach

ABSTRACT: The global sanitary crisis derived from antibiotic multi-resistant bacteria entails the need to reduce sulfamethoxazole (SMX) concentrations in wastewater treatment plants (WWTPs). The key microorganisms and the biotransformation mechanisms leading to SMX removal remain incompletely characterized, particularly under aerobic heterotrophic conditions, which are becoming increasingly relevant in the design of novel, more energy-efficient, WWTPs. In this study, sequential batch reactors were inoculated with activated sludge, operated in heterotrophic conditions and spiked with six different initial SMX concentrations ranging between 0 and 2000 µg L-1. The goal was to determine the influence of SMX in the microbiome and its enzymatic expression through genomic, metaproteomic and transformation product analyses. The results allowed us to identify the metabolite 2,4(1H,3H)-pteridinedione-SMX (PtO-SMX), pointing to the role of the pterin-conjugation pathway in the biotransformation of SMX. Additionally, at increased SMX concentrations, through metaproteomics and 16S rRNA gene sequencing, it was determined a higher abundance of the genus Corynebacterium and a differential expression of five enzymes involved in its central metabolism, suggesting the relevant role of this bacteria to mitigate SMX risks.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Environmental Samples <g-proteobacteria Environmental Samples <b-proteobacteria

SUBMITTER: Valentina Calamia

LAB HEAD: Alba Trueba Santiso

PROVIDER: PXD029711 | Pride | 2022-09-20

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	5-13-2021_SMX_Tf1000_100ng_1603.d.7z	Other
	5-13-2021_SMX_Tf1000_100ng_1603.mgf	Mgf
	5-13-2021_SMX_tf0_100ng_1587.d.7z	Other
	5-13-2021_SMX_tf0_100ng_1587.mgf	Mgf
	5-13-2021_SMX_tf250_100ng_1595.d.7z	Other

Items per page:

1 - 5 of 13

Similar Datasets

Project description:Membrane Vesicles (MVs) are envelope derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied in pathogenic microorganisms, the roles, relevance and biotechnological potential of MVs from environmental bacteria are less well established. Acidithiobacillaceae family bacteria are active players in the sulfur and iron biogeochemical cycles in extreme acidic environments and drivers of the leaching of mineral ores contributing to acid rock/mine drainage (ARD/AMD) and industrial bioleaching. One key aspect to such rol is the ability of these bacteria to tightly interact with the mineral surfaces and extract electrons and nutrients to support their chemolithotrophic metabolism. Despite recent advances in the characterization of acidithiobacilli biofilms and extracellular matrix (ECM) components, our understanding of its architectural and mechanistic aspects remains scant. In this work, we show that vesiculation is a common phenomenon in distant members of the Acidithiobacillaceae family, and further explore the role of MVs in multicellular colonization behaviours using `Fervidacidithiobacillus caldus´ as bacterial model. Production of MVs in `F. caldus´ occurred in both planktonic cultures and biofilms formed on sulfur surfaces, where MVs appeared individually or in chains resembling Tube-Shaped Membranous Structures (TSMSs) important for microbial communication. Liquid chromatography-mass spectrometry data and bioinformatic analysis of the MVs-associated proteome revealed that F. caldus MVs were enriched in proteins involved in cell-cell and cell-surface processes, and largely typified the MVs as outer MVs (OMVs). Finally, microbiological assays showed that amendment of `F. caldus´ MVs to cells and/or biofilms affects collective colonizing behaviours relevant to the ecophysiology and applications of these acidophiles providing grounds for their exploitation in mining biotechnologies.

Dataset Information

Sulfamethoxazole triggers specific microbial activities under aerobic heterotrophic conditions: A metaproteomic approach

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets