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Molecular mechanisms involved in Atlantic halibut egg quality

ABSTRACT: Egg quality is of pivotal importance in biomedicine, agriculture, ecology and environmental science because of its tremendous influence on reproductive success or failure in all animals. Despite species specific differences in physiological aspects of early development, the evolutionary conserved stereotypical procedure of cellular events, led us to investigate whether these findings are common with marine fish species of aquaculture interest. Atlantic halibut (Hippoglossus hippoglossus) is a highly prized species in global fish markets with increasing demand to its production. The objectives of this study were 1) to reveal the proteomic profiles of good versus poor quality halibut eggs, 2) to identify proteins that can serve as egg quality markers, and 3) to discover molecular mechanisms determining egg quality using a combination of quantitative proteomics workflows, tandem mass tags (TMT) labeling and parallel reaction monitoring (PRM).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Hippoglossus Hippoglossus

TISSUE(S): Egg

SUBMITTER: OZLEM YILMAZ

LAB HEAD: Birgitta Norberg

PROVIDER: PXD029894 | Pride | 2022-07-06

REPOSITORIES: Pride

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Project description:Background: Poultry eggs are a low cost, high protein nutrient package that can be consumed as part of a quality diet. However, consumption of poultry egg products has been historically contentious, which highlights the importance of investigating impacts of long-term egg consumption on metabolic health. Objective: Our study utilized the zebrafish Danio rerio, a newly-defined model of human metabolic health, to understand the metabolic consequence of consuming egg products in lieu of other well-described protein sources. Methods: Reference diets were formulated to contain multi-source protein with casein and fish protein hydrolysate (CON; control protein sources), the protein sources of which have been vetted in numerous reference diets. These proteins were then replaced in part with either whole egg (WE; protein and lipid source), egg white (EW; protein source), wheat gluten (WG; cereal protein source), or a high lipid content diet containing a multi-source protein with casein and fish protein hydrolysate (HFCON; isonitrogenous and isolipidic with the WE diet) in a 34-week trial. Daily feeding was initiated at early juvenile life stage and terminated at late reproductive adult stage. Results: The amino acid composition of control vs egg product diets did not vary substantially, although methionine and lysine were apparently limiting in fish fed WG. At termination, fish fed EW as the protein source had similar weight gain and body composition to those fed the CON diet. Blood glucose, both fasting and postprandial, did not differ among any dietary treatment. Assessment of the liver transcriptome using RNAseq revealed no differential gene expression between zebrafish fed CON or WE diets. Zebrafish fed WG had lower weight gain in males. Conclusions: Long-term consumption of egg products promoted metabolic health equal to that of historically-relevant proteins. These data support the value of egg products for maintaining long-term metabolic health in animal diets.

Project description:Molecular profiling of the eggs is an excellent approach aiming at understanding biological processes and mechanisms conditioning developmental competence in finfishes and, consequently, reproductive fitness. Despite many efforts, it is still unclear what is the specific role of transcriptome and proteome in determination of egg quality in Teleostei fishes. The aim of this study was to perform integrated transcriptomic-proteomic analysis of eggs of pikeperch – a commercially relevant freshwater fish species. Additionally, comparative analysis of transcriptome and proteome in eggs characterized by either high or low egg quality has been performed, in order to identify mechanisms leading to early embryonic lethality in pikeperch. Our study provides a novel insight into the understanding of the role of maternally-derived molecular cargo in finfishes. The data obtained sheds light on the importance of transcriptome in development of nervous system suggesting neurogenesis-related mRNAs as a very important, non-genetic inheritance factor. Proteomic analysis highlights specific role of proteins in the immune response in ovulated eggs. Integrated analysis brings attention to the galactose-specific lectin nattectin gene and protein as the frontline defense molecule for the egg and developing embryo. The molecular analysis of egg developmental competence emphasizes post-vitellogenic processes (final oocyte maturation and ovulation) as the ones potentially compromising transcriptomic profile, but not affecting proteomic cargo. It highlights the need for careful reconsideration of the commercial reproductive protocols as the very strong modulators of egg quality and their molecular profile. Considering the mechanisms driving these alterations as well as consequences stemming from the differential abundance of the transcripts are still to be explored, the candidate quality markers, first time provided for pikeperch along with the current study, are creating valuable resource for further studies.

			Action	DRS
	Oz_TMT-F.mzML	Mzml
	Oz_TMT-F.mzid.gz	Mzid
	Oz_TMT-F0.raw	Raw
	Oz_TMT-F1.raw	Raw
	Oz_TMT-F2.raw	Raw

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Molecular mechanisms involved in Atlantic halibut egg quality

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