Dataset Information

Anoplocephala perfoliata whole somatic proteome

ABSTRACT: Equid tapeworms have previously been considered to be of little pathological importance. Consequently, molecular characterisation of the proteome of Anoplocephala perfoliata has been previously neglected. However, associations with significant intestinal damage and colic cases demonstrate A. perfoliata infections pose a greater risk to equine health than previously considered. In the absence of commercial vaccines, the sole control option for equid tapeworms is via anthelmintic treatment. Limitations in control options pose a risk in anthelmintic resistance development and a reduced ability to sustain control of equid tapeworms. Proteomic analysis and characterisation of glutathione transferases (GST) have shown promise as novel anthelmintic or vaccine targets for improved helminth control. So whole worm proteome was analysed through 1DE and LC-MSMS to localise GST in the whole somatic proteome which may not be purified through GSH columns.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Anoplocephala Perfoliata

TISSUE(S): Whole Body

SUBMITTER: Holly Northcote

LAB HEAD: Dr Russell Morphew

PROVIDER: PXD029998 | Pride | 2024-05-22

REPOSITORIES: Pride

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Publications

A dominance of Mu class glutathione transferases within the equine tapeworm Anoplocephala perfoliata.

Northcote Holly M HM Wititkornkul Boontarikaan B Cutress David J DJ Allen Nathan D ND Brophy Peter M PM Wonfor Ruth E RE Morphew Russell M RM

Parasitology 20240111 3

The most common equine tapeworm, Anoplocephala perfoliata, has often been neglected amongst molecular investigations and has been faced with limited treatment options. However, the recent release of a transcriptome dataset has now provided opportunities for in-depth analysis of A. perfoliata protein expression. Here, global, and sub-proteomic approaches were utilized to provide a comprehensive characterization of the A. perfoliata soluble glutathione transferases (GST) (ApGS ...[more]

PMID: 38200699

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Project description:Ascarid parasites infect a variety of hosts, and control measures rely heavily on anthelmintics raising concerns for anthelmintic resistance. Parascaris spp., the equine ascarid, is the only ascarid with documented substantial drug resistance. Elucidating drug responses of susceptible parasites may lead to further understanding of anthelmintic resistance mechanisms, identifying alternative drug targets, and parameters for slowing the development of resistance among other ascarid species. The purpose of this study was to examine the response of anthelmintic naïve Parascaris spp. to in vitro drug exposure using transcriptomic analyses and quantitative PCR. Adult Parascaris spp. worms were obtained at necropsy from foals born into a herd kept without anthelmintic treatment. Adult worms were maintained in Roswell-Park Memorial medium 1640. The drugs employed were oxibendazole (OBZ) and ivermectin (IVM), and 10-fold serial dilutions were prepared. Optimum drug concentration and exposure length were determined using a worm viability assessment method. Subsequently, RNA-seq analysis identified genes with significantly different expression between drug treated and control groups. Finally, a selection of the significant genes was further investigated using qPCR in a repeat drug exposure trial. Following initial viability assessments, OBZ (10 μg/mL) for 24 hours and IVM (1 μg/mL) for three hours were the conditions employed. RNA-seq analysis revealed a total of 88 transcripts with significantly (α=0.01) different expression between all drug treated and control worms. The top five genes selected for further analysis were snap25, cytochrome p450, kelch10, sup9, and ferm. Gene functionalities are related to muscle contraction, detoxification and exocytotic events, and regulatory processes. qPCR did not identify any significant differences between groups, but this may be due to the lack of method sensitivity. The results of RNA-seq analysis and gene function warrant further investigation and their role in ascarid xenobiotic responses.

			Action	DRS
	C1A.mgf	Mgf
	C1A.raw	Raw
	C1B.mgf	Mgf
	C1B.raw	Raw
	C1C.mgf	Mgf

Dataset Information

Anoplocephala perfoliata whole somatic proteome

Dataset's files

Publications

A dominance of Mu class glutathione transferases within the equine tapeworm <i>Anoplocephala perfoliata</i>.

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