Dataset Information

Identification of phosphorylation sites of transcription factor after reaction with protein kinase

ABSTRACT: In previous study, we reported that MdERF17 contributes to Chl degradation in apple fruit peels. Here, we demonstrated that MdERF17 and MdMPK4 interact with each other in vitro and in vivo and raised the possibility that MdMPK4 may contribute to fruit degreening via post-transcriptional regulation of MdERF17. So an in vitro kinase assay was conducted and LC-MS/MS was used to examine the phosphorylation sites.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Malus Domestica

SUBMITTER: Luping Zheng

LAB HEAD: Shuai Wang

PROVIDER: PXD030037 | Pride | 2022-05-20

REPOSITORIES: Pride

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Publications

Phosphorylation of MdERF17 by MdMPK4 promotes apple fruit peel degreening during light/dark transitions.

Wang Shuai S Wang Ting T Li Qiqi Q Xu Chen C Tian Ji J Wang Yi Y Zhang Xinzhong X Xu Xuefeng X Han Zhenhai Z Wu Ting T

The Plant cell 20220401 5

As apple fruits (Malus domestica) mature, they accumulate anthocyanins concomitantly with losing chlorophyll (Chl); however, the molecular pathways and events that coordinate Chl degradation and fruit coloration have not been elucidated. We showed previously that the transcription factor ETHYLENE RESPONSE FACTOR17 (MdERF17) modulates Chl degradation in apple fruit peels and that variation in the pattern of MdERF17 serine (Ser) residues is responsible for differences in its transcriptional regula ...[more]

PMID: 35166845

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Project description:Molecular events regulating apple fruit ripening and sensory quality are largely unknown. Such knowledge is essential for genomic-assisted apple breeding and postharvest quality management. In this study, a parallel transcriptome profile analysis, scanning electron microscopic (SEM) examination and systematic physiological characterization were performed on two apple cultivars, Honeycrisp (HC) and Cripps Pink (CP), which have distinct ripening features and texture attributes. Systematic physiological characterization of fruit ripening based on weekly maturity data indicated substantial differences in fruit crispness and firmness at comparable ripening stages. SEM images of fruit cortex tissues prepared from fruits with equivalent maturity suggested that the cell wall thickness may contribute to the observed phenotypes of fruit firmness and crispness. A high-density long-oligo apple microarray consisting of duplex 190,135 cross-hybridization-free 50-70-mer isothermal probes, and representing 23,997 UniGene clusters, was manufactured on a Nimblegen array platform. Transcriptome profiling identified a total of 1793 and 1209 UniGene clusters differentially expressed during ripening from cortex tissues of HC and CP, respectively. UniGenes implicated in hormone metabolism and response, cell wall biosynthesis and modification and those encoding transcription factors were among the prominent functional groups. Between the two cultivars, most of the identified UniGenes were similarly regulated during fruit ripening; however, a short list of gene families or specific family members exhibited distinct expression patterns between the two cultivars, which may represent candidate genes regulating cultivar-specific apple fruit ripening patterns and quality attributes. Using a single color labeling system, a total of 24 microarray slides were utilized, one for each cortex tissue sample, for transcriptome profiling analysis. 2 cultivars x 3 developmental stages x 4 biological replicates.

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	19_4-3.mgf	Mgf
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