Dataset Information

Aggregation under genotoxic conditions in HEK293T and U2OS cells

ABSTRACT: This dataset consists of 2 independent experiments. The first research aim was to identify the proteins that aggregate in HEK293T cells treated with the chemotherapeutic agent camptothecin (CPT), the second to identify the proteins that aggregate in U2OS cells after a loss of the checkpoint kinase ATM. For the first experiment, cells were treated for 24 hours with vehicle (DMSO) or CPT, left to recover for 48 hours, and harvested. For the second experiment, wild-type and ATM KO cells were grown for 72 hours and harvested. For both experiments, aggregated proteins were isolated by a differential detergent fractionation protocol, which ultimately resulted in a 1% SDS insoluble protein fraction. These proteins were solubilized in 8M urea.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Bone Cancer

SUBMITTER: Luciano Di Stefano

LAB HEAD: John LaCava

PROVIDER: PXD030166 | Pride | 2022-04-08

REPOSITORIES: Pride

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Publications

Targeting DNA topoisomerases or checkpoint kinases results in an overload of chaperone systems, triggering aggregation of a metastable subproteome.

Huiting Wouter W Dekker Suzanne L SL van der Lienden Joris C J JCJ Mergener Rafaella R Musskopf Maiara K MK Furtado Gabriel V GV Gerrits Emma E Coit David D Oghbaie Mehrnoosh M Di Stefano Luciano H LH Schepers Hein H van Waarde-Verhagen Maria A W H MAWH Couzijn Suzanne S Barazzuol Lara L LaCava John J Kampinga Harm H HH Bergink Steven S

eLife 20220224

A loss of the checkpoint kinase ataxia telangiectasia mutated (ATM) leads to impairments in the DNA damage response, and in humans causes cerebellar neurodegeneration, and an increased risk of cancer. A loss of ATM is also associated with increased protein aggregation. The relevance and characteristics of this aggregation are still incompletely understood. Moreover, it is unclear to what extent other genotoxic conditions can trigger protein aggregation as well. Here, we show that targeting ATM, ...[more]

PMID: 35200138

			Action	DRS
	10_DMSO_SDS_3_5uL_20200612085223.raw	Raw
	11_Cpt_wcl_3.raw	Raw
	11_WCL_ATM.raw	Raw
	12_Cpt_SDS_3_5uL.raw	Raw
	13_DMSO_wcl_4.raw	Raw

Dataset Information

Aggregation under genotoxic conditions in HEK293T and U2OS cells

Dataset's files

Publications

Targeting DNA topoisomerases or checkpoint kinases results in an overload of chaperone systems, triggering aggregation of a metastable subproteome.

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