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Aggregation under genotoxic conditions in HEK293T and U2OS cells

ABSTRACT: This dataset consists of 2 independent experiments. The first research aim was to identify the proteins that aggregate in HEK293T cells treated with the chemotherapeutic agent camptothecin (CPT), the second to identify the proteins that aggregate in U2OS cells after a loss of the checkpoint kinase ATM. For the first experiment, cells were treated for 24 hours with vehicle (DMSO) or CPT, left to recover for 48 hours, and harvested. For the second experiment, wild-type and ATM KO cells were grown for 72 hours and harvested. For both experiments, aggregated proteins were isolated by a differential detergent fractionation protocol, which ultimately resulted in a 1% SDS insoluble protein fraction. These proteins were solubilized in 8M urea.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Bone Cancer

SUBMITTER: Luciano Di Stefano

LAB HEAD: John LaCava

PROVIDER: PXD030166 | Pride | 2022-04-08

REPOSITORIES: pride

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			Action	DRS
	10_DMSO_SDS_3_5uL_20200612085223.raw	Raw
	11_Cpt_wcl_3.raw	Raw
	11_WCL_ATM.raw	Raw
	12_Cpt_SDS_3_5uL.raw	Raw
	13_DMSO_wcl_4.raw	Raw

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