Proteomics

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Exploring the molecular mechanism of dihydroartemisinin regulating spleen immunity based on proteomics and phosphoproteomic


ABSTRACT: The molecular mechanisms through which dihydroartemisinin (DHA) induces immunomodulation in vivo remain unclear. Herein, DHA-regulated proteins and their phosphorylation profiles were identified and characterized, in the spleens of mice, using proteomics and phosphoproteomic approaches. We found that DHA upregulates proliferation-associated protein (cyclin-dependent kinases, Ki67, and mini-chromosome maintenance and proliferating cell nuclear antigen) expression via modulation of mitogen-activated protein kinase (MAPK) - activator protein 1 (AP-1) signaling pathway activity. In addition, DHA promoted the proliferation of CD4 T naïve, proliferated CD25+CD4+ T-cells, and BrdU+ interferon (IFN)-γ-producing CD8+ T cells. These predicted mechanisms of action were then validated in a Plasmodium berghei ANKA-infected mouse model of malaria and a cyclophosphamide-induced immunosuppression model. Collectively, the findings of this study, for the first time, provide robust evidence that DHA induced the expansion of the splenic T-cell pool through MAPK-AP1 signaling. The data provided in-depth knowledge in the mechanism of DHA-mediated immunomodulation.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen

SUBMITTER: qilong li  

LAB HEAD: Qijun Chen

PROVIDER: PXD030353 | Pride | 2022-05-29

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
SD085LPST_CMC_1_Slot2-34_1_2285.d.zip Other
SD085LPST_CMC_2_Slot2-34_1_2287.d.zip Other
SD085LPST_CMC_3_Slot2-34_1_2289.d.zip Other
SD085LPST_DHA_1_Slot2-33_1_2279.d.zip Other
SD085LPST_DHA_2_Slot2-33_1_2281.d.zip Other
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