Dataset Information

Label-free quantitative proteome of AML CD34+ and mobilized PB CD34+ cells

ABSTRACT: The label-free quantitative proteome was generated for 42 primary AML patient samples enriched for CD34+ cells (or mononuclear cells in the case of NPMcyt sameples) and as controls 6 mobilized peripheral blood CD34+ cells were included. Furthermore, 6 AML cell lines were included, and also primary mesenchymal stem cells grown under normaoxia or hypoxia were included.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Bone Marrow, Blood

DISEASE(S): Acute Leukemia

SUBMITTER: Jan Jacob Schuringa

LAB HEAD: Jan Jacob Schuringa

PROVIDER: PXD030463 | Pride | 2022-03-15

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Sample_and_Data_Relationship_Format_SDRF_file.txt	Txt
	Schuringa_AML_global_proteomics-proteinGroups_log10_normalized.xlsx	Xlsx
	Schuringa_AML_global_proteomics-proteinGroups_log10_normalized_LFQ_intensities.xlsx	Xlsx

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Publications

Prospective Isolation and Characterization of Genetically and Functionally Distinct AML Subclones.

de Boer Bauke B Prick Janine J Pruis Maurien G MG Keane Peter P Imperato Maria Rosaria MR Jaques Jennifer J Brouwers-Vos Annet Z AZ Hogeling Shanna M SM Woolthuis Carolien M CM Nijk Marije T MT Diepstra Arjan A Wandinger Sebastian S Versele Matthias M Attar Ricardo M RM Cockerill Peter N PN Huls Gerwin G Vellenga Edo E Mulder André B AB Bonifer Constanze C Schuringa Jan Jacob JJ

Cancer cell 20180920 4

Intra-tumor heterogeneity caused by clonal evolution is a major problem in cancer treatment. To address this problem, we performed label-free quantitative proteomics on primary acute myeloid leukemia (AML) samples. We identified 50 leukemia-enriched plasma membrane proteins enabling the prospective isolation of genetically distinct subclones from individual AML patients. Subclones differed in their regulatory phenotype, drug sensitivity, growth, and engraftment behavior, as determined by RNA seq ...[more]

PMID: 30245083

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