Proteomics

Dataset Information

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Swine Acute Diarrhea Syndrome Coronavirus Induces Autophagy to Promote Its Replication via the Akt/mTOR Pathway


ABSTRACT: Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an enveloped, single‐stranded, positive‐sense RNA viruse belonging to Coronaviridae family. An increasing number of studies have demonstrated that viruses could utilize autophagy to promote their own replication. However, the relationship between SADS-CoV and autophagy machinery remained unknown. Here, we reported that SADS-CoV infection induced autophagy in cultured cells and pharmacologically increased autophagy was conducive to viral proliferation. Conversely, suppression of autophagy by pharmacological inhibitor or knockdown of autophagy-related protein impeded viral replication. Furthermore, we demonstrated the underlying mechanisms by which SADS-CoV triggered autophagy through inactivation of the Akt/mTOR pathway. Importantly, we identified integrin α3 (ITGA3) as a potential antiviral target upstream of Akt/mTOR and autophagy pathways by analyzing the proteomic profiles. Knockdown of ITGA3 using RNA inference activated autophagy and as a consequence, increased the replication of SADS-CoV. Collectively, our studies revealed a novel mechanism that SADS-CoV induced autophagy to facilitate its proliferation via Akt/mTOR pathway and found that ITGA3 is an effective antiviral factor for suppressing viral infection.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Chlorocebus Sabaeus

TISSUE(S): Cell Culture, Nephrocyte

DISEASE(S): Diarrhea

SUBMITTER: YU XIA  

LAB HEAD: Yongchang Cao

PROVIDER: PXD030559 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

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Action DRS
XM_HD_20210216_21P00580001.raw Raw
XM_HD_20210216_21P00580002.raw Raw
XM_HD_20210216_21P00580003.raw Raw
XM_HD_20210216_21P00580011.raw Raw
XM_HD_20210216_21P00580012.raw Raw
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Publications

Swine acute diarrhea syndrome coronavirus induces autophagy to promote its replication via the Akt/mTOR pathway.

Zeng Siying S   Zhao Yan Y   Peng Ouyang O   Xia Yu Y   Xu Qiuping Q   Li Hongmei H   Xue Chunyi C   Cao Yongchang Y   Zhang Hao H  

iScience 20221020 11


Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an enveloped, single-stranded, positive-sense RNA virus belonging to the Coronaviridae family. Increasingly studies have demonstrated that viruses could utilize autophagy to promote their own replication. However, the relationship between SADS-CoV and autophagy remains unknown. Here, we reported that SADS-CoV infection-induced autophagy and pharmacologically increased autophagy were conducive to viral proliferation. Conversely, suppression  ...[more]

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