Dataset Information

Two closely related white rot fungi, Obba rivulosa and Gelatoporia subvermispora, produce orthologous plant cell wall degrading enzymes for spruce wood degradation

ABSTRACT: Unique ability of basidiomycete white rot fungi to degrade all components of plant cell walls makes them indispensable organisms in global carbon cycle. In this study, we analyzed proteomes of two closely related white rot fungi, Obba rivulosa and Gelatoporia subvermispora, while growing on solid spruce wood, and defined a core set of CAZymes that was shared between these species including the orthologous enzymes. Similar production pattern of these CAZymes indicate their key role in plant biomass degradation and need for their further biochemical characterization. The obtained results give an insight into specific enzymes and enzyme sets that are produced during the degradation of solid spruce wood. These findings expand the knowledge on enzyme production in nature-mimicking conditions and may contribute to exploitation of white rot fungi and their enzymes in biotechnological applications.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Ceriporiopsis Subvermispora Obba Rivulosa

TISSUE(S): Fungus, Fungal Cell

SUBMITTER: Marcos Di Falco

LAB HEAD: Kristiina Sari Hildén

PROVIDER: PXD030609 | Pride | 2022-08-08

REPOSITORIES: Pride

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Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora.

Marinovíc Mila M Di Falco Marcos M Aguilar Pontes Maria Victoria MV Gorzsás András A Tsang Adrian A de Vries Ronald P RP Mäkelä Miia R MR Hildén Kristiina K

Biomolecules 20220722 8

The unique ability of basidiomycete white rot fungi to degrade all components of plant cell walls makes them indispensable organisms in the global carbon cycle. In this study, we analyzed the proteomes of two closely related white rot fungi, Obba rivulosa and Gelatoporia subvermispora, during eight-week cultivation on solid spruce wood. Plant cell wall degrading carbohydrate-active enzymes (CAZymes) represented approximately 5% of the total proteins in both species. A core set of o ...[more]

PMID: 35892327

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Project description:Fomitiporia mediterranea (Fmed) is one of the main fungal species found in grapevine wood rot, also called “amadou”, one of the most typical symptoms of grapevine trunk disease Esca. This fungus is functionally classified as a white-rot, able to degrade all wood structure polymers, i.e., hemicelluloses, cellulose, and the most recalcitrant component, lignin. Specific enzymes are secreted by the fungus to degrade those components, namely carbohydrate active enzymes for hemicelluloses and cellulose, which can be highly specific for given polysaccharide, and peroxidases, which enable white-rot to degrade lignin, with specificities relating to lignin composition as well. Furthermore, besides polymers, a highly diverse set of metabolites often associated with antifungal activities is found in wood, this set differing among the various wood species. Wood decayers possess the ability to detoxify these specific extractives and this ability could reflect the adaptation of these fungi to their specific environment. The aim of this study is to better understand the molecular mechanisms used by Fmed to degrade wood structure, and in particular its potential adaptation to grapevine wood. To do so, Fmed was cultivated on sawdust from different origins: grapevine, beech, and spruce. Carbon mineralization rate, mass loss, wood structure polymers contents, targeted metabolites and secreted proteins were measured. We used the well-known white-rot model Trametes versicolor for comparison. Whereas no significant degradation was observed with spruce, a higher mass loss was measured on Fmed grapevine culture compared to beech culture. Moreover, on both substrates, a simultaneous degradation pattern and the degradation of wood extractives were demonstrated, and proteomic analyses identified a relative overproduction of oxidoreductases involved in lignin and extractive degradation on grapevine cultures, and only few differences in carbohydrate active enzymes. These results could explain at least partially the adaptation of Fmed to grapevine wood structural composition compared to other wood species and suggest that other biotic and abiotic factors should be considered to fully understand the potential adaptation of Fmed to its ecological niche.

			Action	DRS
	Cersu_Data_file_information.xlsx	Xlsx
	MM03072015_C2A.raw	Raw
	MM03072015_C4A.raw	Raw
	MM03072015_C8A.raw	Raw
	MM03072015_CersuDB-FullTryp.msf	Msf

Dataset Information

Two closely related white rot fungi, Obba rivulosa and Gelatoporia subvermispora, produce orthologous plant cell wall degrading enzymes for spruce wood degradation

Dataset's files

Publications

Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: <i>Obba rivulosa</i> and <i>Gelatoporia subvermispora</i>.

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