Dataset Information

A nuclear receptor facilitates differentiation of human PSCs into more mature hepatocytes

ABSTRACT: To start examining the mechanisms by which THRB mediated the epigenetic changes, we performed immunoprecipitation (IP) of THRB in THRB-FLAG HepG2 cells with anti-FLAG antibody, followed by mass-spectrometry to identify THRB-binding proteins.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

DISEASE(S): Liver Cancer

SUBMITTER: Haiting Ma

LAB HEAD: Rudolf Jaenisch

PROVIDER: PXD030665 | Pride | 2022-03-25

REPOSITORIES: Pride

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			Action	DRS
	210914_19_3haiting_IgG-Control.mgf	Mgf
	210914_19_3haiting_IgG-Control.mzxml	Mzxml
	210914_19_3haiting_IgG-Control.raw	Raw
	210914_19_3haiting_IgG-Control_peptides.pep.xml	Pepxml
	210914_19_3haiting_IgG-Control_peptides_1_1_0.mzid.gz	Mzid

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Publications

The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes.

Ma Haiting H de Zwaan Esmée E Guo Yang Eric YE Cejas Paloma P Thiru Prathapan P van de Bunt Martijn M Jeppesen Jacob F JF Syamala Sudeepa S Dall'Agnese Alessandra A Abraham Brian J BJ Fu Dongdong D Garrett-Engele Carrie C Lee Tong Ihn TI Long Henry W HW Griffith Linda G LG Young Richard A RA Jaenisch Rudolf R

Cell stem cell 20220421 5

To understand the mechanisms regulating the in vitro maturation of hPSC-derived hepatocytes, we developed a 3D differentiation system and compared gene regulatory elements in human primary hepatocytes with those in hPSC-hepatocytes that were differentiated in 2D or 3D conditions by RNA-seq, ATAC-seq, and H3K27Ac ChIP-seq. Regulome comparisons showed a reduced enrichment of thyroid receptor THRB motifs in accessible chromatin and active enhancers without a reduced transcription of THRB. The addit ...[more]

PMID: 35452598

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