Dataset Information

Apoptotic auditory brainstem of E13 chick embryo

ABSTRACT: We characterized the proteome of the auditory brainstem of a chick embryo on embryonic day 13, when apoptosis occurs in auditory nuclei. We identified caspase substrates by searching the peptidome for peptides C-terminal to caspase-typical cleavage sites.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Brain

SUBMITTER: Forrest Weghorst

LAB HEAD: Karina Cramer

PROVIDER: PXD030697 | Pride | 2022-04-01

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	2021--10-20-Cramer-E13A-100_211021162555.raw	Raw
	2021--10-20-Cramer-E13A-130.raw	Raw
	2021--10-20-Cramer-E13A-176.raw	Raw
	2021--10-20-Cramer-E13A-395_211022185400.raw	Raw
	2021--10-20-Cramer-E13A-54.raw	Raw

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Publications

Non-Apoptotic Caspase Activity Preferentially Targets a Novel Consensus Sequence Associated With Cytoskeletal Proteins in the Developing Auditory Brainstem.

Weghorst Forrest F Mirzakhanyan Yeva Y Hernandez Kiersten L KL Gershon Paul D PD Cramer Karina S KS

Frontiers in cell and developmental biology 20220307

The auditory brainstem relies on precise circuitry to facilitate sound source localization. In the chick, the development of this specialized circuitry requires non-apoptotic activity of caspase-3, for which we previously identified several hundred proteolytic substrates. Here we tested whether the sequence of the caspase cleavage site differentially encodes proteolytic preference in apoptotic and non-apoptotic contexts. We constructed a consensus sequence for caspase activity in the non-apoptot ...[more]

PMID: 35330912

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