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Comparison of wild type and abc1k6-1 mutant total leaf extracts from 4 and 5 week old plants

ABSTRACT: The quantitative proteomes of leaf tissue from A. thaliana at 4 weeks and 5 weeks of growth using tandem mass spectrometry with label-free quantification. A comparison of three genotypes has been performed, namely i) the Col-0 wild type ecotype, ii) a T-DNA insertion mutant disrupting the plastoglobule-localized ABC1K6 protein (abc1k6-1) that is delayed in transition to reproductive growth, and iii) a complementation line (Comp3) that restores the timely progression of development with the ABC1K6 wild-type protein sequence tagged C-terminally with GFP in the abc1k6-1 background. Whereas wild-type and abc1k6-1 remain in the vegetative growth stage at 4 weeks of age, the wild-type plants have transitioned to reproductive growth (as evidenced by the emergence of the infloresence bolt) by 5 weeks of age, while abc1k6-1 remians in vegetative growth. Our proteomics experiment seeks to identify leaf proteome differences prior to wild-type transition to reproductive growth that may account for the delayed transition in the abc1k6-1 mutant.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Peter Lundquist

LAB HEAD: Peter Lundquist

PROVIDER: PXD030750 | Pride | 2022-03-02

REPOSITORIES: Pride

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			Action	DRS
	Araport11_proteins.201606.fasta	Fasta
	File_List_20210412.xlsx	Xlsx
	MaxQuant_Output.zip	Other
	MaxQuant_Software_Package_v1.16.11.0.zip	Other
	checksum.txt	Txt

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