Dataset Information

Murine Western diet-induced nonalcoholic steatohepatitis liver proteome

ABSTRACT: Non-alcoholic fatty liver disease (NAFLD) is the number one cause of chronic liver disease worldwide, with 25% of these patients developing nonalcoholic steatohepatitis (NASH). NASH significantly increases the risk of cirrhosis and decompensated liver failure. Past studies in rodent models have shown the knockout of glycine-N-methyltransferase (GNMT) results in rapid pro-gression of steatosis, fibrosis, and hepatocellular carcinoma. However, the attenuation of GNMT in subjects with NASH and the molecular basis for its impact on the disease process are still unclear. To address this knowledge gap, we show the reduction of GNMT protein levels in the liver of NASH subjects compared to healthy controls. To gain insight into the impact of decreased GNMT in the disease process, we performed global label-free proteome studies on the livers from a murine Western diet-based model of NASH. Histological and molecular characterization of the animal model demonstrate high resemblance to the human disease.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Connor Quinn

LAB HEAD: Salim Merali

PROVIDER: PXD030781 | Pride | 2022-04-04

REPOSITORIES: Pride

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			Action	DRS
	QE_mice_liver_CHOW_0_556_SCX1.raw	Raw
	QE_mice_liver_CHOW_0_556_SCX2.raw	Raw
	QE_mice_liver_CHOW_0_556_SCX3.raw	Raw
	QE_mice_liver_CHOW_0_556_SCX4.raw	Raw
	QE_mice_liver_CHOW_0_556_SCX5.raw	Raw

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Publications

Dysregulation of S-adenosylmethionine Metabolism in Nonalcoholic Steatohepatitis Leads to Polyamine Flux and Oxidative Stress.

Quinn Connor C Rico Mario C MC Merali Carmen C Merali Salim S

International journal of molecular sciences 20220211 4

Nonalcoholic fatty liver disease (NAFLD) is the number one cause of chronic liver disease worldwide, with 25% of these patients developing nonalcoholic steatohepatitis (NASH). NASH significantly increases the risk of cirrhosis and decompensated liver failure. Past studies in rodent models have shown that glycine-<i>N</i>-methyltransferase (GNMT) knockout results in rapid steatosis, fibrosis, and hepatocellular carcinoma progression. However, the attenuation of GNMT in subjects with NASH and the ...[more]

PMID: 35216100

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