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Yeats cells AP-MS for Jhd2 and Tpk2 relative to article Phosphorylation of Jhd2 by the Ras-cAMP- PKA pathway regulates histone modifications and autophagy

ABSTRACT: This work uncovers a novel and biologically significant mechanism that directly connects nutrient availability to histone modifications and gene transcription. We report that glycolysis promotes histone H3K4 trimethylation (H3K4me3) by activating Tpk2, a catalytic subunit of protein kinase A (PKA) via the Ras-cyclic AMP (cAMP) pathway. Glucose-activated PKA (Tpk2) inhibits Jhd2-catalyzed H3K4 demethylation by phosphorylating Jhd2 at serine 321 and serine 340. In addition, Tpk2- catalyzed Jhd2 phosphorylation promotes H3K14ac by preventing the binding of Rpd3 at chromatin.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Yu Qi

LAB HEAD: yu qi

PROVIDER: PXD030815 | Pride | 2022-08-29

REPOSITORIES: Pride

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			Action	DRS
	Jhd2-A.pdResult	Other
	Jhd2-A.pdResultView	Other
	Jhd2.raw	Raw
	Jhs2a-2021.3.24.msf	Msf
	Jhs2a-2021.3.24.msfView	Msf

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Jhd2 S321A S340A mutant Transcriptomes

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