Proteomics

Dataset Information

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Protein expression landscape defines the formation potential of mouse blastoids from EPS cells


ABSTRACT: Preimplantation embryo development is a precisely regulated process organized by maternally inherited and newly synthesized proteins. Recently, some studies have reported that blastocyst-like structures, named blastoids, can be generated from mouse ESCs (embryonic stem cells) or EPSCs (extended pluripotent stem cells). In this study, to explore the dynamic expression characteristics of proteins and their PTMs in mouse EPS blastoids, we revealed the protein expression profile of EPS-blastoids and metabolite characteristics by TMT-based quantitative mass spectrometry (MS) strategy. Furthermore, the protein phosphorylation sites were identified to show the phosphoproteomic analysis in blastoids compared with mouse early embryos. Above all, our study revealed the protein expression profile of EPS blastoids compared with mouse embryos during preimplantation development and indicated that glucose metabolism is key to blastoid formation.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blastocyst

SUBMITTER: Zheying Min  

LAB HEAD: Zheying Min

PROVIDER: PXD031002 | Pride | 2022-04-04

REPOSITORIES: Pride

Dataset's files

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HA022TPST_Fr1.zip Other
HA022TPST_Fr2.zip Other
HA022TPST_Fr3.zip Other
HA022TPST_Fr4.zip Other
HA022TQ_01.zip Other
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Publications

Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs.

Min Zheying Z   Zhong Ke K   Luo Yuxin Y   Fan Yong Y   Yu Yang Y  

Frontiers in cell and developmental biology 20220208


Preimplantation embryo development is a precisely regulated process organized by maternally inherited and newly synthesized proteins. Recently, some studies have reported that blastocyst-like structures, named blastoids, can be generated from mouse ESCs (embryonic stem cells) or EPSCs (extended pluripotent stem cells). In this study, to explore the dynamic expression characteristics of proteins and their PTMs in mouse EPS blastoids, we revealed the protein expression profile of EPS blastoids and  ...[more]

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