Dataset Information

Identification of a HTT-specific binding motif in DNAJB1 essential for suppression and disaggregation of HTT

ABSTRACT: Identification of the binding interface between HttExon1Q48 and the molecular chaperone DNAJB1. The proteins were incubated and cross-linked in vitro and the binding interface was mapped to the hinge region between CTDI and CTDII of DNAJB1 and the proline rich domain of HTTEXon1Q48.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

SUBMITTER: Heike Stephanowitz

LAB HEAD: Janine Kirstein

PROVIDER: PXD031214 | Pride | 2022-10-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	All_crosslinks_spectra_merged.pdf	Pdf
	DNAJB1-HTTQ48_XL.csv	Csv
	F1_20171023_HS785_2.raw	Raw
	F1_20180122_HS_HS819_JK_7_SDAmethod.raw	Raw
	HTTQ23-HSP70_XL.csv	Csv

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Publications

Identification of a HTT-specific binding motif in DNAJB1 essential for suppression and disaggregation of HTT.

Ayala Mariscal S M SM Pigazzini M L ML Richter Y Y Özel M M Grothaus I L IL Protze J J Ziege K K Kulke M M ElBediwi M M Vermaas J V JV Colombi Ciacchi L L Köppen S S Liu F F Kirstein J J

Nature communications 20220810 1

Huntington's disease is a neurodegenerative disease caused by an expanded polyQ stretch within Huntingtin (HTT) that renders the protein aggregation-prone, ultimately resulting in the formation of amyloid fibrils. A trimeric chaperone complex composed of Hsc70, DNAJB1 and Apg2 can suppress and reverse the aggregation of HTTExon1Q<sub>48</sub>. DNAJB1 is the rate-limiting chaperone and we have here identified and characterized the binding interface between DNAJB1 and HTTExon1Q<sub>48</sub>. DNAJB ...[more]

PMID: 35948542

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