Proteomics

Dataset Information

0

Paramecium Polycomb Repressive Complex 2 physically interacts with the small RNA binding PIWI protein to repress transposable elements


ABSTRACT: Polycomb Repressive Complex 2 (PRC2) maintains transcriptionally silent genes in a repressed state via deposition of histone H3 K27 trimethyl (me3) marks. PRC2 has also been implicated in silencing transposable elements (TEs) yet how PRC2 is targeted to TEs remains unclear. To address this question, we performed tandem affinity purification combined with mass spectrometry and identified proteins that physically interact with the Paramecium Enhancer-of-zeste Ezl1 enzyme, which catalyzes H3K9me3 and H3K27me3 deposition at TEs. We show that the Paramecium PRC2 core complex comprises four subunits, each required in vivo for catalytic activity. We also identify PRC2 cofactors, including the RNA interference (RNAi) effector Ptiwi09, which are necessary to target H3K9me3 and H3K27me3 to TEs. We find that the physical interaction between PRC2 and the RNAi pathway is mediated by a RING finger protein and that small RNA recruitment of PRC2 to TEs is analogous to the small RNA recruitment of H3K9 methylation SU(VAR)3-9 enzymes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Paramecium Tetraurelia

TISSUE(S): Cell Culture

SUBMITTER: Laurent Lignières  

LAB HEAD: Guillaume Chevreux

PROVIDER: PXD031335 | Pride | 2022-04-06

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
35830_64366.pep.xml Pepxml
35831_64367.pep.xml Pepxml
35832_64368.pep.xml Pepxml
35833_64369.pep.xml Pepxml
40556_67749.pep.xml Pepxml
Items per page:
1 - 5 of 55

Similar Datasets

2023-04-01 | GSE190308 | GEO
2024-10-17 | PXD045214 | Pride
2024-10-17 | PXD045266 | Pride
2016-01-04 | PXD002282 | Pride
2015-06-25 | E-GEOD-62666 | biostudies-arrayexpress
2023-02-22 | MSV000091349 | MassIVE
2011-09-08 | E-GEOD-30157 | biostudies-arrayexpress
2018-05-07 | PXD005821 | Pride
2020-12-07 | PXD012347 | Pride
2018-11-21 | PXD007154 | Pride