Proteomics

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The structural dynamics of full length divisome transmembrane proteins FtsQ, FtsB, and FtsL in FtsQBL complex formation


ABSTRACT: Bacterial divisome is a group of proteins governing cell division. Out of the 15 critical proteins in Escherichia coli, the transmembrane protein complex FtsQBL plays an essential role in regulating the action. Although extensive efforts have been made, there is limited information regarding the interaction within this three-member complex. Here, HDX-MS was used to study the dynamic change of the full-length protein FtsQ, FtsB, and FtsL. Direct experimental evidence shows that FtsB and FtsL form a stable complex by the transmembrane and periplasmic regions. After the complex formation, the structural feature in FtsB properly becomes more defined. This transition may also bring the constriction control domains (CCDs) close to the one in FtsL. Furthermore, with the helical interaction between two proteins, the CCDs have a more determined location relative to the membrane surface. On the other hand, the FtsQ in the FtsQBL complex showed rigidity enhancement in two regions. The interaction with FtsB would fix a significant portion of the FtsQ β-domains. Interestingly, the formation of the FtsQBL complex also stiffens the sequence connecting the two domains in FtsQ. Overall, this study provides important experimental evidence on the conformational dynamic change of the FtsQ, FtsB, and FtsL upon the complex formation and insights into the FtsQBL function in the divisome.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Escherichia Coli

SUBMITTER: Wai Po Kong  

LAB HEAD: Kwok-yin Wong

PROVIDER: PXD031342 | Pride | 2022-10-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20181123_FtsQ_1h_1.raw.zip Raw
20181123_FtsQ_1h_2.raw.zip Raw
20181123_FtsQ_1h_3.raw.zip Raw
20181123_FtsQ_1min_1.raw.zip Raw
20181123_FtsQ_1min_2.raw.zip Raw
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Publications

The structural dynamics of full-length divisome transmembrane proteins FtsQ, FtsB, and FtsL in FtsQBL complex formation.

Kong Wai-Po WP   Gong Furong F   So Pui-Kin PK   Chen Yu Wai YW   Chan Pak-Ho PH   Leung Yun-Chung YC   Wong Kwok-Yin KY  

The Journal of biological chemistry 20220704 8


FtsQBL is a transmembrane protein complex in the divisome of Escherichia coli that plays a critical role in regulating cell division. Although extensive efforts have been made to investigate the interactions between the three involved proteins, FtsQ, FtsB, and FtsL, the detailed interaction mechanism is still poorly understood. In this study, we used hydrogen-deuterium exchange mass spectrometry to investigate these full-length proteins and their complexes. We also dissected the structural dynam  ...[more]

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