Dataset Information

NanoTACs: NanoLuc-Targeting Protein Degraders that Parallel Existing Technology

ABSTRACT: NanoLuc-targeting PROTACs (NanoTACs) hijack the CRBN and VHL cullin-RING ligase complexes to trigger proteasomal degradation of substrates tagged with the 19 kDa luminescent NanoLuc tag. In order to validate the system and confirm selective degradation of the target protein and assess any potential off-target degradation, here DDA-PASEF label-free quantification was performed to monitor target-induced degradation following addition of NC4.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Simon Cobbold

LAB HEAD: Simon Cobbold

PROVIDER: PXD031371 | Pride | 2022-05-20

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PROTAC_10_1_1_4823.d.zip	Other
	PROTAC_1_1_1_4830.d.zip	Other
	PROTAC_2_1_1_4821.d.zip	Other
	PROTAC_3_1_1_4824.d.zip	Other
	PROTAC_4_1_1_4810.d.zip	Other

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1 - 5 of 11

Publications

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.

Grohmann Christoph C Magtoto Charlene M CM Walker Joel R JR Chua Ngee Kiat NK Gabrielyan Anna A Hall Mary M Cobbold Simon A SA Mieruszynski Stephen S Brzozowski Martin M Simpson Daniel S DS Dong Hao H Dorizzi Bridget B Jacobsen Annette V AV Morrish Emma E Silke Natasha N Murphy James M JM Heath Joan K JK Testa Andrea A Maniaci Chiara C Ciulli Alessio A Lessene Guillaume G Silke John J Feltham Rebecca R

Nature communications 20220419 1

Modulation of protein abundance using tag-Targeted Protein Degrader (tTPD) systems targeting FKBP12<sup>F36V</sup> (dTAGs) or HaloTag7 (HaloPROTACs) are powerful approaches for preclinical target validation. Interchanging tags and tag-targeting degraders is important to achieve efficient substrate degradation, yet limited degrader/tag pairs are available and side-by-side comparisons have not been performed. To expand the tTPD repertoire we developed catalytic NanoLuc-targeting PROTACs (NanoTACs) ...[more]

PMID: 35440107

Dataset Information

NanoTACs: NanoLuc-Targeting Protein Degraders that Parallel Existing Technology

Dataset's files

Publications

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.

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