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Biosynthesis of polycyclopropanated high energy biofuels

ABSTRACT: Cyclopropanes-functionalized hydrocarbons are excellent fuels. however, their synthesis is challenging and harmful for the environment. In this work we produced polycyclopropanated fatty acids in bacteria. These molecules can be easily converted into renewable fuels for high energy applications such as shipping, long-haul transport, aviation, and rocketry. We explored the chemical diversity encoded in the genome of thousands of bacteria to identify and repurpose naturally occurring cyclopropanated molecules. We identified a set of candidate iterative Polyketide Synthases (iPKSs) predicted to produce polycyclopropanated fatty acids (POP-FAs), expressed these PKSs in Streptomyces coelicolor and produced the POP-FAs. We determined the structure of the molecules and increased their production 22-fold. Polycyclopropanated fatty acid methyl esters (POP-FAMEs) were obtained by methyl esterifying the POP-FAs. Finally, we calculated the enthalpy of combustion of several POP fuel candidates to assess their potential as replacement for fossil fuels in energy demanding applications. Our research shows that POP-FAMEs and other polyketide derived POPs have the energetic properties for energy demanding applications for which sustainable alternatives are scarce.

INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS

ORGANISM(S): Escherichia Coli

SUBMITTER: Christopher Petzold

LAB HEAD: Chris Petzold

PROVIDER: PXD031402 | Pride | 2022-09-19

REPOSITORIES: Pride

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	checksum.txt	Txt
	ecoli_1.d.zip	Other
	ecoli_1__F019181_.mzid.gz	Mzid
	ecoli_1__F019181_.mzid_ecoli_1__F019181_.MGF	Mzid
	ecoli_4.d.zip	Other

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