Proteomics

Dataset Information

0

Different roles of gamma- and beta-actin in neuronal functions


ABSTRACT: Lack of cytoplasmic beta-actin gene (Actb) leads to early embryonic lethality in mice, however targeted editing of Actb coding sequence by introducing five point mutations to encode cytoplasmic gamma-actin protein does not affect mouse viability. These mice with beta to gamma actin replacement (Actbc-g mice) develop normally and show no detectable phenotypes at young age, despite complete lack of beta-actin protein. Here we investigated the effect of the replacement of beta-actin with gamma-actin in the brain and the retina --- the tissue where these two cytoplasmic actin isoforms predominate.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Dawei Dong  

LAB HEAD: Dawei W Dong

PROVIDER: PXD031462 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
KashinaA-19-G120-Sample1-QEP.raw Raw
KashinaA-19-G120-Sample10-QEP.raw Raw
KashinaA-19-G120-Sample11-QEP.raw Raw
KashinaA-19-G120-Sample12-QEP.raw Raw
KashinaA-19-G120-Sample13-QEP.raw Raw
Items per page:
1 - 5 of 18
altmetric image

Publications

Differential N-terminal processing of beta and gamma actin.

Chen Li L   Vedula Pavan P   Tang Hsin Yao HY   Dong Dawei W DW   Kashina Anna S AS  

iScience 20220923 10


Cytoplasmic beta- and gamma-actin are ubiquitously expressed in every eukaryotic cell. They are encoded by different genes, but their amino acid sequences differ only by four conservative substitutions at the N-termini, making it difficult to dissect their individual regulation. Here, we analyzed actin from cultured cells and tissues by mass spectrometry and found that beta, unlike gamma actin, undergoes sequential removal of N-terminal Asp residues, leading to truncated actin species found in b  ...[more]

Similar Datasets

2023-03-11 | PXD031465 | Pride
2024-02-19 | PXD039786 | Pride
2023-03-11 | PXD032086 | Pride
2023-03-11 | PXD032139 | Pride
2023-03-11 | PXD031481 | Pride
2023-04-24 | PXD040174 | Pride
2020-08-26 | GSE156817 | GEO
2023-05-24 | GSE185078 | GEO
2014-06-05 | E-GEOD-56461 | biostudies-arrayexpress
2019-09-11 | GSE103718 | GEO