Dataset Information

The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts

ABSTRACT: Sporozoites and merozoites share a number of proteins that are expressed by both stages, including the Apical Membrane Antigen 1 (AMA1) and the Rhoptry Neck Proteins (RONs). Although AMA1 and RONs are essential for merozoite invasion of erythrocytes during asexual blood stage replication of the malaria parasite, their precise function in sporozoites is unclear. Here we performed RFP-trap immunoprecipitation experiments using lysates from transgenic sporozoites expressing RON4 fused to mCherry. RON4, RON2, RON5 and AMA1 were the main proteins identified by mass spectrometry among co-precipitated proteins, showing that AMA1-RON interactions are conserved in salivary gland sporozoites.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Plasmodium Berghei Anka

DISEASE(S): Malaria

SUBMITTER: Soumia HAMADA

LAB HEAD: Solenne Chardonnet

PROVIDER: PXD031463 | Pride | 2022-08-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Anopheles_stephensi_UP.fasta	Fasta
	PlasmoDB_PB_39_PbergheiANKA.fasta	Fasta
	ProteoPB_1DE09_IP-R1_Slot1-21_01_3745.d.zip	Other
	ProteoPB_1DE09_IP-R1_Slot1-21_01_3745_DA5.1.201_1.mgf	Mgf
	ProteoPB_1DE09_IP-R1_Slot1-21_01_3745_DA5.xml	Xml

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Publications

The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts.

Fernandes Priyanka P Loubens Manon M Le Borgne Rémi R Marinach Carine C Ardin Béatrice B Briquet Sylvie S Vincensini Laetitia L Hamada Soumia S Hoareau-Coudert Bénédicte B Verbavatz Jean-Marc JM Weiner Allon A Silvie Olivier O

PLoS pathogens 20220622 6

Plasmodium sporozoites that are transmitted by blood-feeding female Anopheles mosquitoes invade hepatocytes for an initial round of intracellular replication, leading to the release of merozoites that invade and multiply within red blood cells. Sporozoites and merozoites share a number of proteins that are expressed by both stages, including the Apical Membrane Antigen 1 (AMA1) and the Rhoptry Neck Proteins (RONs). Although AMA1 and RONs are essential for merozoite invasion of erythrocytes durin ...[more]

PMID: 35731833

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