Proteomics

Dataset Information

0

Cross-linking mass spectrometry and integrative modelling of Methanocaldococcus jannaschii GMP synthetase


ABSTRACT: GMP synthetases are enzymes that catalyze the conversion of XMP to GMP. The two-subunit type GMP synthetases are composed of a glutamine amidotransferase (GATase) subunit that catalyzes the conversion of Gln to Glu and ammonia, and the ATP pyrophosphatase (ATPPase) subunit that catalyzes the formation of AMP-XMP from ATP and XMP. The inactive GATase subunit is allosterically activated by the binding of substrates to the ATPPase subunit. Upon activation, the GATase subunit binds Gln and hydrolyzes it producing ammonia which is tunnelled to the ATPPase subunit. The two subunits form a tight complex to enable domain crosstalk. However, the Methanocaldococcus jannaschii GMP synthetase (MjGMPS) is unique as the GATase (MjGATase) and ATPPase (MjATPPase) subunits interact transiently. Here, we employed enzyme kinetics, X-ray crystallography, cross-linking mass spectrometry (XL-MS) and integrative modelling to understand the mechanistic basis for the various steps in the catalytic cycle of MjGMPS.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Methanocaldococcus Jannaschii Dsm 2661

SUBMITTER: Santosh Shivakumaraswamy  

LAB HEAD: Hemalatha Balaram

PROVIDER: PXD031519 | Pride | 2022-09-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AB1_01.raw Raw
AB1_02.raw Raw
AB1_04.raw Raw
AB1_05.raw Raw
AB1_06.raw Raw
Items per page:
1 - 5 of 33
altmetric image

Publications

Mechanistic Insights into the Functioning of a Two-Subunit GMP Synthetase, an Allosterically Regulated, Ammonia Channeling Enzyme.

Shivakumaraswamy Santosh S   Kumar Sanjeev S   Bellur Asutosh A   Polisetty Satya Dev SD   Balaram Hemalatha H  

Biochemistry 20220830 18


Guanosine 5'-monophosphate (GMP) synthetases, enzymes that catalyze the conversion of xanthosine 5'-monophosphate (XMP) to GMP, are composed of two different catalytic units, which are either two domains of a polypeptide chain or two subunits that associate to form a complex. The glutamine amidotransferase (GATase) unit hydrolyzes glutamine generating ammonia, and the ATP pyrophosphatase (ATPPase) unit catalyzes the formation of an AMP-XMP intermediate. The substrate-bound ATPPase allosterically  ...[more]

Similar Datasets

2020-02-04 | PXD014673 | Pride
2022-12-02 | PXD037049 | Pride
2020-04-22 | PXD017792 | Pride
2021-09-10 | PXD023525 | Pride
2021-05-21 | PXD019048 | Pride
2023-10-11 | PXD045446 | Pride
2023-01-25 | PXD030619 | Pride
2020-05-26 | PXD010177 | Pride
2023-03-10 | PXD033966 | Pride
2019-01-08 | PXD009260 | Pride