Dataset Information

GPR56 (ADGRG1) and Transglutaminase (TG2) interaction in Regulation of Mesenchymal Transition in Glioblastoma.

ABSTRACT: G protein-coupled receptor 56 (GPR56/ADGRG1) is an adhesion GPCR with an essential role in brain development and cancer. Elevated expression of GPR56 was observed in the clinical specimens of Glioblastoma (GBM), a highly invasive primary brain tumor. However, we found the expression to be variable across the specimens, presumably due to the intratumor heterogeneity of GBM. Therefore, we re-examined GPR56 expression in public domain spatial gene expression data and single-cell expression data for GBM, which revealed that GPR56 expression was high in cellular tumors, infiltrating tumor cells, and proliferating cells, low in microvascular proliferation and peri-necrotic areas of the tumor, especially in hypoxic mesenchymal-like cells. To gain a better understanding of the consequences of GPR56 downregulation in tumor cells and other molecular changes associated with it, we generated a sh-RNA-mediated control and GPR56 knockdown in the GBM cell line U373 and performed transcriptomics, proteomics, and phospho-proteomics analysis and using the data we propose a putative model to explain this functional and regulatory relationship of the two proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Leukocyte, Sperm, Photosynthetic Cell, Permanent Cell Line Cell, Melanocyte, Epithelial Cell, Embryonic Stem Cell, Plant Cell, Malignant Cell, Multipotent Stem Cell, Hepatocyte, Keratinocyte, B Cell, Monocyte, Electrically Active Cell, Stem Cell, Diploid Cell, Erythrocyte, Platelet, T Cell, Dendritic Cell, Early Embryonic Cell, Neutrophil, Fibroblast, Oocyte, Macrophage

DISEASE(S): Glioblastoma

SUBMITTER: Ravi Sirdeshmukh