PT-modified B12 probe and Non-PT-modified B34 probe pull-down with Salmonella enterica serovar Cerro 87 cell lysate
Ontology highlight
ABSTRACT: To capture proteins that exhibit differential binding with PT- and non-PT-modified DNA in vivo, we performed pull-down assays using biotinylated DNA probes and the cell lysate of S. enterica serovar Cerro 87 that contains the dndBCDE-dndFGH module. For these experiments, we used two 30 bp DNA probes, B12 and B34, which share the same DNA sequences but possess PT-modified 5’-GPSAAC-3’/5’-GPSTTC-3’ and non-PT-modified 5’-GAAC-3’/5’-GTTC-3’ motifs, respectively.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Cerro
SUBMITTER: You Tang
LAB HEAD: You Tang
PROVIDER: PXD031869 | Pride | 2022-02-26
REPOSITORIES: Pride
ACCESS DATA