Proteomics

Dataset Information

0

Plasma samples processed with depletion and non depletion


ABSTRACT: Plasma samples processed with no depletion and most-abundant-proteins depletion procedures according to standard in-solution and single-pot, solid-phase-enhaced sample preparation (SP3) workflows using guanidyne hydrochloride and SDS lysis buffers for analysis with liquid chromatography mass spectrometry (LC-MS).

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Maria Hernandez-Valladares  

LAB HEAD: Frode Berven

PROVIDER: PXD031919 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Comparing Efficiency of Lysis Buffer Solutions and Sample Preparation Methods for Liquid Chromatography-Mass Spectrometry Analysis of Human Cells and Plasma.

Neset Lasse L   Takayidza Gracious G   Berven Frode S FS   Hernandez-Valladares Maria M  

Molecules (Basel, Switzerland) 20220525 11


The use of a proper sample processing methodology for maximum proteome coverage and high-quality quantitative data is an important choice to make before initiating a liquid chromatography-mass spectrometry (LC-MS)-based proteomics study. Popular sample processing workflows for proteomics involve in-solution proteome digestion and single-pot, solid-phase-enhanced sample preparation (SP3). We tested them on both HeLa cells and human plasma samples, using lysis buffers containing SDS, or guanidiniu  ...[more]

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