Proteomics profiling of mitotic arrest by transcriptional silencing of the Cdc20 homolog slp1 in fission yeast

ABSTRACT: Depletion of the APC/C activator Cdc20 arrests cells in metaphase with high levels of the mitotic cyclin (Cyclin B) and the Separase inhibitor Securin. In mammalian cells this arrest has been exploited for the treatment of cancer with drugs that engage the spindle assembly checkpoint and, recently, with chemical inhibitors of the APC/C. While most cells arrested in mitosis for prolonged periods undergo apoptosis, others skip cytokinesis and enter G1 with unsegregated chromosomes. This process, known as mitotic slippage, generates aneuploidy and increases genomic instability in the cancer cell. Here, we analyse the behaviour of fission yeast cells arrested in mitosis through the transcriptional silencing of the Cdc20 homolog slp1. While depletion of slp1 readily halts cells in metaphase, this arrest is only transient and a majority of cells eventually undergo cytokinesis and show steady mitotic dephosphorylation. As a result, we generated this label-free dataset with the aim of providing further insights into the global dynamics of protein expression under slp1 depletion and the underlying mechanisms that contribute to the mitotic escape.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Schizosaccharomyces Pombe 972h-

SUBMITTER: Nathalia Chica  

LAB HEAD: Sandra Lopez-Aviles

PROVIDER: PXD031975 | Pride | 2022-10-13

REPOSITORIES: Pride