Proteomics

Dataset Information

0

SARS-CoV-2 ORF8 interactome in the secretory pathway


ABSTRACT: The replication machinery of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is closely associated with the endoplasmic reticulum (ER) in host cells. Activation of the unfolded protein response (UPR) is a strategy hijacked by coronavirus to facilitate its replication and suppress host innate immunity. Here, we identified that many protein disulfide isomerase family members are enriched in the ORF8 interactome, and found that ORF8 protein accumulates in the ER and escapes the degradation system by forming mixed disulfide complexes with ER oxidoreductases. ORF8 induces the activation of three UPR pathways through targeting key UPR components, remodels ER morphology and accelerates protein trafficking.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jifeng Wang  

LAB HEAD: Wang Lei

PROVIDER: PXD032035 | Pride | 2022-08-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Ctrl_B_the_second_dimension.raw Raw
Ctrl_S_the_second_dimension.raw Raw
Ctrl_the_first_dimension.raw Raw
LFQ_the_first_dimension.msf Msf
LFQ_the_second_dimension.msf Msf
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Publications

SARS-CoV-2 ORF8 reshapes the ER through forming mixed disulfides with ER oxidoreductases.

Liu Ping P   Wang Xi X   Sun Yiwei Y   Zhao Hongyu H   Cheng Fang F   Wang Jifeng J   Yang Fuquan F   Hu Junjie J   Zhang Hong H   Wang Chih-Chen CC   Wang Lei L  

Redox biology 20220628


The replication machinery of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is closely associated with the endoplasmic reticulum (ER) in host cells. Activation of the unfolded protein response (UPR) is a strategy hijacked by coronavirus to facilitate its replication and suppress host innate immunity. Here, we have found that SARS-CoV-2 ORF8 protein accumulates in the ER and escapes the degradation system by forming mixed disulfide complexes with ER oxidoreductases. ORF8 induces  ...[more]

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