Dataset Information

Characterization of the skeletal muscle proteome in under-nourished old rats

ABSTRACT: Therefore, undernutrition is common in the elderly where its prevalence varies from 4 to 10% at home, from 15 to 30% in care homes and reaches 30 to 70% in hospitals de-pending on the diagnostic criteria used [10]. Most of all, aging impairs the ability to adapt to undernutrition leading to severe loss of body weight and muscle [11-14]. In particular, old rats exhibit defective adaptation to long-term 50% dietary restriction initiated at an advanced age, resulting in dramatic body weight loss and deregulation of protein metabolism in terms of nitrogen balance and protein content, especially at skeletal muscle level [12]. Therefore, in elderly patients, undernutrition exacerbates the state of frailty and dependence, greatly increasing the risk of morbidity and mortality [15]. This is even more critical that the world aging population is growing up. Accord-ing to United Nations statistics on the demographic trend in aging population, the proportion of persons aged 65 or over in the world is projected to reach nearly 16% in 2050, compared to 9% in 2019 [16]. Specific studies are needed to better understand the undernutrition-induced skeletal muscle alterations in order to identify new therapeu-tic targets and prevent sarcopenia in the old population

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Soleus

SUBMITTER: christophe Chambon

LAB HEAD: PFEM composante proteomique

PROVIDER: PXD032044 | Pride | 2022-06-09

REPOSITORIES: Pride

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Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats.

Barbé Caroline C Salles Jérôme J Chambon Christophe C Giraudet Christophe C Sanchez Phelipe P Patrac Véronique V Denis Philippe P Boirie Yves Y Walrand Stéphane S Gueugneau Marine M

International journal of molecular sciences 20220426 9

Aging is associated with a progressive loss of skeletal muscle mass and function termed sarcopenia. Various metabolic alterations that occur with aging also increase the risk of undernutrition, which can worsen age-related sarcopenia. However, the impact of undernutrition on aged skeletal muscle remains largely under-researched. To build a deeper understanding of the cellular and molecular mechanisms underlying age-related sarcopenia, we characterized the undernutrition-induced changes in the sk ...[more]

PMID: 35563153

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Project description:Background: Age-related physiological, biochemical and functional changes in mammalian skeletal muscle have been shown to begin at the mid-point of the lifespan. However, the underlying changes in DNA methylation that occur during this turning point of the muscle aging process have not been clarified. To explore age-related genomic methylation changes in skeletal muscle, we employed young (0.5 years old) and middle-aged (7 years old) pigs as models to survey genome-wide DNA methylation in the longissimus dorsi muscle using a methylated DNA immunoprecipitation sequencing approach. Results: We observed a tendency toward a global loss of DNA methylation in the gene-body region of the skeletal muscle of the middle-aged pigs compared with the young group. We determined the genome-wide gene expression pattern in the longissimus dorsi muscle using microarray analysis and performed a correlation analysis using DMR (differentially methylated region)-mRNA pairs, and we found a significant negative correlation between the changes in methylation levels within gene bodies and gene expression. Furthermore, we identified numerous genes that show age-related methylation changes that are potentially involved in the aging process. The methylation status of these genes was confirmed using bisulfite sequencing PCR. The genes that exhibited a hypomethylated gene body in middle-aged pigs were over-represented in various proteolysis and protein catabolic processes, suggesting an important role for these genes in age-related muscle atrophy. In addition, genes associated with tumorigenesis exhibited aged-related differences in methylation and expression levels, suggesting an increased risk of disease associated with increased age. Conclusions: This study provides a comprehensive analysis of genome-wide DNA methylation patterns in aging pig skeletal muscle. Our findings will serve as a valuable resource in aging studies, promoting the pig as a model organism for human aging research and accelerating the development of comparative animal models in aging research. We collected the longissimus dorsi muscles tissue from Jinhua pigs which aged 0.5 year and seven years and study the genome-wide DNA methylation difference between the two age periods.

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