Proteomics

Dataset Information

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Detecting mistranslation from a serine tRNA with proline anticodon expressed in yeast


ABSTRACT: Mistranslation, the mis-incorporation of an amino acid not specified by the “standard” genetic code, occurs in all cells. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. The goal of this study was to detect and quantify mistranslation from a serine tRNA variant with proline UGG anticodon and G26A secondary mutation engineered in yeast

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Matthew Berg  

LAB HEAD: Judit Villen

PROVIDER: PXD032063 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Genetic background and mistranslation frequency determine the impact of mistranslating tRNASerUGG.

Berg Matthew D MD   Zhu Yanrui Y   Loll-Krippleber Raphaël R   San Luis Bryan-Joseph BJ   Genereaux Julie J   Boone Charles C   Villén Judit J   Brown Grant W GW   Brandl Christopher J CJ  

G3 (Bethesda, Md.) 20220701 7


Transfer RNA variants increase the frequency of mistranslation, the misincorporation of an amino acid not specified by the "standard" genetic code, to frequencies approaching 10% in yeast and bacteria. Cells cope with these variants by having multiple copies of each tRNA isodecoder and through pathways that deal with proteotoxic stress. In this study, we define the genetic interactions of the gene encoding tRNASerUGG,G26A, which mistranslates serine at proline codons. Using a collection of yeast  ...[more]

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