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Identifying interaction proteins of METTL16 using co immunoprecipitation and mass spectroscopy

ABSTRACT: METTL16 has recently been identified as an RNA methyltransferase that installs m6A marks on a few transcripts. But its function in cytosol remains unclear. Puromycin-labelling and renilla luciferase assay revealed that METTL16 can promote translation efficiency in an m6A-independent manner. To explore the mechanism, we performed co-immunoprecipitation using METTL16 antibody and mass spectroscopy to identify its interaction proteins. Moreover, we find that METTL16 is essential for tumorigenesis of non-small cell lung cancer.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Xue Mi

LAB HEAD: Jun Zhou

PROVIDER: PXD032068 | Pride | 2024-08-03

REPOSITORIES: Pride

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			Action	DRS
	480_FPEP200624411-1A.raw	Raw
	480_FPEP200624411-1A_PeptideGroups.txt	Txt
	480_FPEP200624411-1A_Proteins.txt	Txt
	480_FPEP200624412-1A.raw	Raw
	480_FPEP200624412-1A_PeptideGroups.txt	Txt

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