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Arabidopsis thaliana PATLN 2 interactome

ABSTRACT: Import of ferrous iron (Fe2+) via the plasma membrane ZIP transporter IRON-REGULATED TRANSPORTER1 (IRT1) bears the risk of oxidative damage and lipid peroxidation. The plasma membrane-associated SEC14-like lipid transfer protein PATL2 interacts with the large cytosolic loop and variable region of IRT1 (IRT1vr). PATL2 affects Fe reduction and reactive oxygen species-related responses in roots and alleviates lipid peroxidation upon Fe acquisition. PATL2 binds α-tocopherol, an antioxidant of the vitamin E group.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Gereon Poschmann

LAB HEAD: Gereon Poschmann

PROVIDER: PXD032079 | Pride | 2023-04-25

REPOSITORIES: Pride

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Publications

SEC14-GOLD protein PATELLIN2 binds IRON-REGULATED TRANSPORTER1 linking root iron uptake to vitamin E.

Hornbergs Jannik J Montag Karolin K Loschwitz Jennifer J Mohr Inga I Poschmann Gereon G Schnake Anika A Gratz Regina R Brumbarova Tzvetina T Eutebach Monique M Angrand Kalina K Fink-Straube Claudia C Stühler Kai K Zeier Jürgen J Hartmann Laura L Strodel Birgit B Ivanov Rumen R Bauer Petra P

Plant physiology 20230501 1

Organisms require micronutrients, and Arabidopsis (Arabidopsis thaliana) IRON-REGULATED TRANSPORTER1 (IRT1) is essential for iron (Fe2+) acquisition into root cells. Uptake of reactive Fe2+ exposes cells to the risk of membrane lipid peroxidation. Surprisingly little is known about how this is avoided. IRT1 activity is controlled by an intracellular variable region (IRT1vr) that acts as a regulatory protein interaction platform. Here, we describe that IRT1vr interacted with peripheral plasma mem ...[more]

PMID: 36493393

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