Dataset Information

Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling

ABSTRACT: Viruses must effectively remodel host cellular pathways to replicate and evade immune defenses, and they must do so with limited genomic coding capacity. Targeting post-translational modification (PTM) pathways provides a mechanism by which viruses can broadly and rapidly transform a hostile host environment into a hospitable one. We used mass spectrometry-based proteomics to quantify changes in protein abundance and two PTM types – phosphorylation and ubiquitination – in response to HIV-1 infection with viruses harboring targeted deletions of a subset of HIV-1 genes. PTM analysis revealed a requirement for Aurora kinase activity in HIV-1 infection and identified substrates of a phosphatase that is degraded during infection. Finally, we demonstrated that the Cullin4A-DDB1-DCAF1 E3 ubiquitin ligase ubiquitinates histone H1 somatic isoforms and that the HIV-1 Vpr protein inhibits this process, leading to defects in DNA repair.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

DISEASE(S): Human Immunodeficiency Virus Infectious Disease

SUBMITTER: Jeffrey Johnson

LAB HEAD: Jeffrey Johnson

PROVIDER: PXD032085 | Pride | 2022-04-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20191010.SwissProt.Hsapiens.fasta	Fasta
	20210211_115300_20210210-PA3-ab_Report.xls	Xls
	20210215_184029_EC20210122_Report-modacc.txt	Txt
	20210215_214209_EC20210204_Report-modacc.txt	Txt
	EC20210118_01.raw	Raw

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Publications

Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling.

Johnson Jeffrey R JR Crosby David C DC Hultquist Judd F JF Kurland Andrew P AP Adhikary Prithy P Li Donna D Marlett John J Swann Justine J Hüttenhain Ruth R Verschueren Erik E Johnson Tasha L TL Newton Billy W BW Shales Michael M Simon Viviana A VA Beltrao Pedro P Frankel Alan D AD Marson Alexander A Cox Jeffery S JS Fregoso Oliver I OI Young John A T JAT Krogan Nevan J NJ

Cell reports 20220401 2

Viruses must effectively remodel host cellular pathways to replicate and evade immune defenses, and they must do so with limited genomic coding capacity. Targeting post-translational modification (PTM) pathways provides a mechanism by which viruses can broadly and rapidly transform a hostile host environment into a hospitable one. We use mass spectrometry-based proteomics to quantify changes in protein abundance and two PTM types-phosphorylation and ubiquitination-in response to HIV-1 infection ...[more]

PMID: 35417684

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